Antibodies, Proteins, Kits and Reagents for Life Science

278 083 ₸ Product size

100 µl 278 083 ₸

Order now and get it on Tuesday March 02, 2021

Anti-VAMP2 antibody [EPR20818] (ab215721)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR20818] to VAMP2
Suitable for: IP, IHC-P, WB
Reacts with: Mouse, Rat, Human

Product name

Anti-VAMP2 antibody [EPR20818]
See all VAMP2 primary antibodies
Description

Rabbit monoclonal [EPR20818] to VAMP2
Host species

Rabbit

Tested Applications & Species

Application	Species
IHC-P	Mouse Rat Human
IP	Mouse
WB	Mouse Rat Human

See all applications and species data

Immunogen

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: Neuro-2a, U-87 MG, SH-SY5Y, NIH/3T3, RAW 264.7, PC-12 and C6 whole cell lysates; Human cerebellum and fetal brain lysates; Mouse brain, hippocampus and cerebellum lysates; Rat heart, hippocampus and cerebellum lysates. IHC-P: Human kidney tissue; Mouse and rat cerebrum tissues. IP: Mouse brain lysate.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 0.05% BSA, 40% Glycerol (glycerin, glycerine), PBS
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR20818
Isotype

IgG
Research areas

Western blot - Anti-VAMP2 antibody [EPR20818] (ab215721)

All lanes : Anti-VAMP2 antibody [EPR20818] (ab215721) at 1/1000 dilution

Lane 1 : Mouse brain lysate
Lane 2 : Mouse hippocampus lysate
Lane 3 : Mouse cerebellum lysate
Lane 4 : Neuro-2a (mouse neuroblastoma cell line), whole cell lysate
Lane 5 : PC-12 (rat adrenal gland pheochromocytoma cell line), whole cell lysate
Lane 6 : C6 (rat glial tumor cell line), whole cell lysate
Lane 7 : Rat cerebellum lysate
Lane 8 : Rat hippocampus lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Developed using the ECL technique.

Predicted band size: 12 kDa
Observed band size: 18 kDa
why is the actual band size different from the predicted?

Exposure times: Lanes 1-3, 7-8: 1 second; Lanes 4-5: 3 minutes.

Blocking/Dilution buffer: 5% NFDM/TBST.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VAMP2 antibody [EPR20818] (ab215721)

Immunohistochemical analysis of paraffin-embedded human kidney tissue labeling VAMP2 with ab215721 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Cytoplasmic staining on human kidney. Counter stained with hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunoprecipitation - Anti-VAMP2 antibody [EPR20818] (ab215721)

VAMP2 was immunoprecipitated from 0.35 mg mouse brain lysate with ab215721 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab215721 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10,000 dilution

Lane 1: Mouse brain lysate 10 μg (Input).

Lane 2: ab215721 IP in mouse brain lysate.

Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab215721 in mouse brain lysate.

Exposure time: 1 second.

Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Western blot - Anti-VAMP2 antibody [EPR20818] (ab215721)

All lanes : Anti-VAMP2 antibody [EPR20818] (ab215721) at 1/1000 dilution

Lane 1 : Rat heart lysate
Lane 2 : Human fetal brain lysate
Lane 3 : U-87 MG (human glioblastoma-astrocytoma epithelial cell line), whole cell lysate
Lane 4 : SH-SY5Y (human neuroblastoma cell line from bone marrow), whole cell lysate
Lane 5 : NIH/3T3 (mouse embryonic fibroblast cell line), whole cell lysate
Lane 6 : RAW 264.7 (mouse macrophage cell line transformed with Abelson murine leukemia virus), whole cell lysate
Lane 7 : Human cerebellum lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Developed using the ECL technique.

Predicted band size: 12 kDa
Observed band size: 18 kDa why is the actual band size different from the predicted?

Exposure times: Lanes 1,3-6: 3 minutes; Lanes 2,7: 1 second.

Blocking/Dilution buffer: 5% NFDM/TBST.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VAMP2 antibody [EPR20818] (ab215721)

Immunohistochemical analysis of paraffin-embedded mouse cerebrum tissue labeling VAMP2 with ab215721 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Cytoplasmic staining on mouse cerebrum. Counter stained with hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VAMP2 antibody [EPR20818] (ab215721)

Immunohistochemical analysis of paraffin-embedded rat cerebrum tissue labeling VAMP2 with ab215721 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Cytoplasmic staining on rat cerebrum. Counter stained with hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Anti-VAMP2 antibody [EPR20818] (ab215721)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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