Anti-VAChT antibody (ab235201)
Key features and details
- Rabbit polyclonal to VAChT
- Suitable for: WB, ICC/IF, IHC-P
- Reacts with: Mouse, Rat
- Isotype: IgG
Overview
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Product name
Anti-VAChT antibody
See all VAChT primary antibodies -
Description
Rabbit polyclonal to VAChT -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IF, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Rat
Predicted to work with: Human
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- ICC/IF: Post-natal rat pup (PND1) heterogeneous brain cells. IHC-P: Rat spinal cord tissue. WB: Post-natal rat pup whole brain lysate (PND2-6)
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
Affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunocytochemistry/ Immunofluorescence - Anti-VAChT antibody (ab235201)
4% PFA-fixed, 0.3%Triton X-100 permeabilized Post-natal rat pup (PND1) heterogeneous brain cells labeling VAChT
(green) using ab235201 at 15 μg/mL, O/N at 4⁰C. Donkey Anti-Rabbit IgG DyLight™ 488 Conjugated Preadsorbed was used as the secondary antibody at 5μg/ml for 1 h at RT. The nuclear counterstain was DAPI (blue).Expected localization: predicted membrane, golgi apparatus
Antibody detecting dendrites and axons and specific organelles in the nucleus (nucleolus). Staining correspondent to confirmed VAChT (Slc18a3) staining in mouse brain P7 Gensat project:
http://www.gensat.org/imagenavigator.jsp?imageID=66611
(A) DAPI
(B) ab235201 + secondary antibody
(C) Merge images A+B.
(D) Secondary antibody only
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Western blot - Anti-VAChT antibody (ab235201)Anti-VAChT antibody (ab235201) at 1/1000 dilution + Post-natal rat pup whole brain lysate (PND2-6)
Predicted band size: 56 kDa
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VAChT antibody (ab235201)Paraffin-embedded rat spinal cord tissue stained for VAChT using ab235201 at 1/100 dilution for 30 minutes at room temperature. Secondary antibody was an anti-Rabbit Poly-HRP-IgG, ready to use for 8 minutes at room temperature. Counterstained with hematoxylin.
A: Rat spinal cord H&E.
B: ab235201 20X magnification.
C: ab235201 40X magnification.
