Anti-Vaccinia Virus antibody (ab26853)
Key features and details
- Rabbit polyclonal to Vaccinia Virus
- Suitable for: ELISA
- Reacts with: Vaccinia virus
- Isotype: IgG
Overview
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Product name
Anti-Vaccinia Virus antibody
See all Vaccinia Virus primary antibodies -
Description
Rabbit polyclonal to Vaccinia Virus -
Host species
Rabbit -
Tested applications
Suitable for: ELISAmore details -
Species reactivity
Reacts with: Vaccinia virus -
Immunogen
Synthetic peptide containing amino acids on the predicted N terminal of fusion protein (A27L) in vaccinia virus.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
Storage buffer
Constituent: Whole serum -
Concentration information loading... -
Purity
Whole antiserum -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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ELISA - Anti-Vaccinia Virus antibody (ab26853)ELISA method:
Antigen is coated on EIA strips at 1µg per well.
Add 200µl blocking buffer then wash wells with PBST.
Antiserum or peptide specific purified antibody is diluted in series as 102~106 folds.
Incubate antibody for 1 hour.
Wash unbound antibodies and add HRP-conjugated anti-rabbit IgG.
Wash plates and add substrate to develop color for 5 minutes.
Read absorbance at 650nm. Amount of colour is directly proportional to amount of antibodies.
Antibody titer is defined as >0.1 of absorbance of antiserum minus pre-immune serum.
Titer ~ 1/437000.