Anti-USP39 antibody (ab101902)
Key features and details
- Rabbit polyclonal to USP39
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-USP39 antibody
See all USP39 primary antibodies -
Description
Rabbit polyclonal to USP39 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Synthetic peptide corresponding to Human USP39 aa 450-550 conjugated to keyhole limpet haemocyanin.
(Peptide available asab115141) -
Positive control
- This antibody gave a positive signal in the following cell lysates: HEK293; HeLa; HeLa Nuclear; U20S; Jurkat.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab101902 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
GuaranteedTested applications are guaranteed to work and covered by our Abpromise guarantee.
PredictedPredicted to work for this combination of applications and species but not guaranteed.
IncompatibleDoes not work for this combination of applications and species.
Application Species WB HumanAll applications MouseRatZebrafishMacaque monkeyOrangutanApplication Abreviews Notes WB Use a concentration of 1 µg/ml. Detects a band of approximately 70 kDa (predicted molecular weight: 65 kDa).Notes WB
Use a concentration of 1 µg/ml. Detects a band of approximately 70 kDa (predicted molecular weight: 65 kDa).Target
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Function
May play a role in mRNA splicing. It is unsure if the protein really exhibits hydrolase activity. Could be a competitor of ubiquitin C-terminal hydrolases (UCHs). -
Sequence similarities
Belongs to the peptidase C19 family.
Contains 1 UBP-type zinc finger. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 10713 Human
- Entrez Gene: 28035 Mouse
- Entrez Gene: 100173398 Orangutan
- Entrez Gene: 297336 Rat
- Omim: 611594 Human
- SwissProt: Q53GS9 Human
- SwissProt: Q3TIX9 Mouse
- SwissProt: Q5R761 Orangutan
see all -
Alternative names
- 65K antibody
- CGI 21 antibody
- CGI-21 antibody
see all
Images
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Western blot - Anti-USP39 antibody (ab101902)All lanes : Anti-USP39 antibody (ab101902) at 1 µg/ml
Lane 1 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
Lane 2 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 3 : HeLa (Human epithelial carcinoma cell line) Nuclear Lysate
Lane 4 : U2OS (Human osteosarcoma cell line) Whole Cell Lysate
Lane 5 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 65 kDa
Observed band size: 70 kDa why is the actual band size different from the predicted?
Additional bands at: 20 kDa, 22 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 8 minutes
Datasheets and documents
References (0)
ab101902 has not yet been referenced specifically in any publications.
Images
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Western blot - Anti-USP39 antibody (ab101902)All lanes : Anti-USP39 antibody (ab101902) at 1 µg/ml
Lane 1 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
Lane 2 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 3 : HeLa (Human epithelial carcinoma cell line) Nuclear Lysate
Lane 4 : U2OS (Human osteosarcoma cell line) Whole Cell Lysate
Lane 5 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 65 kDa
Observed band size: 70 kDa why is the actual band size different from the predicted?
Additional bands at: 20 kDa, 22 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 8 minutes
