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Anti-USP14/TGT antibody [EPR15943] - BSA and Azide free (ab251149)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR15943] to USP14/TGT - BSA and Azide free
  • Suitable for: WB, ICC, Flow Cyt
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-USP14/TGT antibody [EPR15943] - BSA and Azide free
    See all USP14/TGT primary antibodies

  • Description

    Rabbit monoclonal [EPR15943] to USP14/TGT - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, ICC, Flow Cytmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    ab251149 is the carrier-free version of ab192618 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    Ab251149 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product was previously labelled as USP14

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunocytochemistry - Anti-USP14/TGT antibody [EPR15943] - BSA and Azide free (ab251149)
    Immunocytochemistry - Anti-USP14/TGT antibody [EPR15943] - BSA and Azide free (ab251149)
    This data was developed using ab192618, the same antibody clone in a different buffer formulation.Immunofluorescent analysis of HeLa cells (4% Paraformaldehyde-fixed, 0.1% tritonX-100 permeabilized) labeling USP14/TGT with ab192618 at 1/70 dilution (5 μg/ml). A Goat anti rabbit IgG (Alexa Fluor488) at 1/400 dilution (ab150077) was used as secondary antibody. Counterstain: DAPI.
  • Flow Cytometry - Anti-USP14/TGT antibody [EPR15943] - BSA and Azide free (ab251149)
    Flow Cytometry - Anti-USP14/TGT antibody [EPR15943] - BSA and Azide free (ab251149)
    This data was developed using ab192618, the same antibody clone in a different buffer formulation.Flow cytometric analysis of Jurkat cells (2% paraformaldehyde-fixed) labeling USP14/TGT with ab192618 at 1/70 dilution (red) or a rabbit IgG (negative) (black), unlabeled cells (blue) followed by Goat anti rabbit IgG (FITC) secondary at 1/150 dilution.
  • Western blot - Anti-USP14/TGT antibody [EPR15943] - BSA and Azide free (ab251149)
    Western blot - Anti-USP14/TGT antibody [EPR15943] - BSA and Azide free (ab251149)
    All lanes : Anti-USP14/TGT antibody [EPR15943] - C-terminal (ab192618) at 1/2000 dilution

    Lane 1 : HeLa cell lysate
    Lane 2 : 293T cell lysate
    Lane 3 : Jurkat cell lysate
    Lane 4 : HCT-116 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

    Predicted band size: 56 kDa



    This data was developed using ab192618, the same antibody clone in a different buffer formulation.

  • Western blot - Anti-USP14/TGT antibody [EPR15943] - BSA and Azide free (ab251149)
    Western blot - Anti-USP14/TGT antibody [EPR15943] - BSA and Azide free (ab251149)
    All lanes : Anti-USP14/TGT antibody [EPR15943] - C-terminal (ab192618) at 1/2000 dilution

    Lane 1 : C6 cell lysate
    Lane 2 : RAW 264.7 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

    Predicted band size: 56 kDa



    This data was developed using ab192618, the same antibody clone in a different buffer formulation.

     

  • Anti-USP14/TGT antibody [EPR15943] - BSA and Azide free (ab251149)
    Anti-USP14/TGT antibody [EPR15943] - BSA and Azide free (ab251149)

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