Anti-ULP-1 antibody (ab34668)
Key features and details
- Rabbit polyclonal to ULP-1
- Suitable for: WB
- Reacts with: Saccharomyces cerevisiae
- Isotype: IgG
Overview
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Product name
Anti-ULP-1 antibody -
Description
Rabbit polyclonal to ULP-1 -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Saccharomyces cerevisiae -
Immunogen
Recombinant full length protein corresponding to Saccharomyces cerevisiae ULP-1 aa 1-697.
Database link: Q09353
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride -
Concentration information loading... -
Purity
IgG fraction -
Purification notes
This product is an IgG fraction antibody purified from monospecific antiserum by a multi-step process including delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against buffer. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-ULP-1 antibody (ab34668)Anti-ULP-1 antibody (ab34668) at 1/1000 dilution + Saccharomyces cerevisiae truncated ULP 1 fusion protein (arrow-head) with 5% non-fat dry milk in TTBS
Secondary
1:1,000 dilution of HRP-labeled
Donkey anti-Rabbit IgG for 1 hour at room temperature.
Predicted band size: 80 kDa
Increasing concentrations of ULP 1 were run on SDS-PAGE and transferred onto nitrocellulose. A chemiluminescence system was used for signal detection (Roche) using a 3 seconds exposure time.