Anti-UBQLN2 antibody [6H9] (ab190283)
![Anti-UBQLN2 antibody [6H9] (ab190283)](https://www.abcam.com/ps/products/190/ab190283/Images/ab190283-313370-anti-ubqln2-antibody-6h9-immunofluorescence.jpg "Anti-UBQLN2 antibody [6H9] (ab190283)")
Key features and details
- Mouse monoclonal [6H9] to UBQLN2
- Suitable for: ICC/IF, WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG1
Overview
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Product name
Anti-UBQLN2 antibody [6H9]
See all UBQLN2 primary antibodies -
Description
Mouse monoclonal [6H9] to UBQLN2 -
Host species
Mouse -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF MouseHumanWB Mouse
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Immunogen
Recombinant fragment corresponding to Human UBQLN2. Expressed in and purified from E.coli.
Database link: Q9UHD9 -
Positive control
- WB: NIH/3T3, C6, HEK-293, HeLa and SH-SY5Y cell lysates; Rat and mouse whole brain lysates; HeLa and 3T3 cell lysates; Transfected primary mouse neuron and glia cell cultures. ICC/IF: NIH/3T3 and HeLa cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.03% Sodium azide
Constituents: PBS, 50% Glycerol -
Concentration information loading... -
Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
6H9 -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Images
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Immunocytochemistry/ Immunofluorescence - Anti-UBQLN2 antibody [6H9] (ab190283)
NIH/3T3 (mouse embyro fibroblast cell line) cells stained for UBQLN2 (green) using ab190283 at 1/1000 dilution in ICC/IF. Costained with chicken pAb to lamin A/C at 1/5,000 dilution (red). The blue is DAPI staining of nuclear DNA.
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![Western blot - Anti-UBQLN2 antibody [6H9] (ab190283)](https://www.abcam.com/ps/products/190/ab190283/Images/ab190283-313369-anti-ubqln2-antibody-6h9-western-blot.jpg)
Western blot - Anti-UBQLN2 antibody [6H9] (ab190283)All lanes : Anti-UBQLN2 antibody [6H9] (ab190283) at 1/1000 dilution
Lane 1 : NIH/3T3 (mouse embyro fibroblast cell line) cell lysate
Lane 2 : C6 (rat glial tumor cell line) cell lysate
Lane 3 : HEK-293 (human epithelial cell line from embryonic kidney) cell lysate
Lane 4 : HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate
Lane 5 : SH-SY5Y (human neuroblastoma cell line from bone marrow) cell lysate
Lane 6 : Rat whole brain lysate
Lane 7 : Mouse whole brain lysate
Predicted band size: 66 kDa
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![Western blot - Anti-UBQLN2 antibody [6H9] (ab190283)](https://www.abcam.com/ps/products/190/ab190283/Images/ab190283-228487-anti-ubqln2-antibody-6h9-western-blot.jpg)
Western blot - Anti-UBQLN2 antibody [6H9] (ab190283)All lanes : Anti-UBQLN2 antibody [6H9] (ab190283) at 1/1000 dilution
Lane 1 : Primary mouse neuron and glia cell cultures
Lane 2 : Primary mouse neuron and glia cells transduced with Human ubiquilin 2 wild type
Lane 3 : Primary mouse neuron and glia cells transduced with ubiquilin 2 P506T mutant
Lane 4 : Primary mouse neuron and glia cells transduced with ubiquilin 2 P497S mutant
Lane 5 : Primary mouse neuron and glia cell cultures transduced with enhanced GFP control
Lane 6 : HeLa cell lysate
Lane 7 : 3T3 cell lysate
Predicted band size: 66 kDa
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![Immunocytochemistry/ Immunofluorescence - Anti-UBQLN2 antibody [6H9] (ab190283)](https://www.abcam.com/ps/products/190/ab190283/Images/ab190283-228486-anti-ubqln2-antibody-6h9-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-UBQLN2 antibody [6H9] (ab190283)Immunofluorescent analysis of HeLa cells labeling UBQLN2 with ab190283 at 1/1000 dilution. Vimentin is stained in red.
