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Anti-UBQLN2 antibody [6H9] (ab190283)

Key features and details

  • Mouse monoclonal [6H9] to UBQLN2
  • Suitable for: ICC/IF, WB
  • Reacts with: Mouse, Rat, Human
  • Isotype: IgG1

Overview

  • Product name

    Anti-UBQLN2 antibody [6H9]
    See all UBQLN2 primary antibodies

  • Description

    Mouse monoclonal [6H9] to UBQLN2

  • Host species

    Mouse

  • Tested Applications & Species

    Application Species
    ICC/IF
    Mouse
    Human
    WB
    Mouse
    See all applications and species data

  • Immunogen

    Recombinant fragment corresponding to Human UBQLN2. Expressed in and purified from E.coli.
    Database link: Q9UHD9

  • Positive control

    • WB: NIH/3T3, C6, HEK-293, HeLa and SH-SY5Y cell lysates; Rat and mouse whole brain lysates; HeLa and 3T3 cell lysates; Transfected primary mouse neuron and glia cell cultures. ICC/IF: NIH/3T3 and HeLa cells.

  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

Images

  • Immunocytochemistry/ Immunofluorescence - Anti-UBQLN2 antibody [6H9] (ab190283)
    Immunocytochemistry/ Immunofluorescence - Anti-UBQLN2 antibody [6H9] (ab190283)

    NIH/3T3 (mouse embyro fibroblast cell line) cells stained for UBQLN2 (green) using ab190283 at 1/1000 dilution in ICC/IF. Costained with chicken pAb to lamin A/C at 1/5,000 dilution (red). The blue is DAPI staining of nuclear DNA. 

  • Western blot - Anti-UBQLN2 antibody [6H9] (ab190283)
    Western blot - Anti-UBQLN2 antibody [6H9] (ab190283)
    All lanes : Anti-UBQLN2 antibody [6H9] (ab190283) at 1/1000 dilution

    Lane 1 : NIH/3T3 (mouse embyro fibroblast cell line) cell lysate
    Lane 2 : C6 (rat glial tumor cell line) cell lysate
    Lane 3 : HEK-293 (human epithelial cell line from embryonic kidney) cell lysate
    Lane 4 : HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate
    Lane 5 : SH-SY5Y (human neuroblastoma cell line from bone marrow) cell lysate
    Lane 6 : Rat whole brain lysate
    Lane 7 : Mouse whole brain lysate

    Predicted band size: 66 kDa

  • Western blot - Anti-UBQLN2 antibody [6H9] (ab190283)
    Western blot - Anti-UBQLN2 antibody [6H9] (ab190283)
    All lanes : Anti-UBQLN2 antibody [6H9] (ab190283) at 1/1000 dilution

    Lane 1 : Primary mouse neuron and glia cell cultures
    Lane 2 : Primary mouse neuron and glia cells transduced with Human ubiquilin 2 wild type
    Lane 3 : Primary mouse neuron and glia cells transduced with ubiquilin 2 P506T mutant
    Lane 4 : Primary mouse neuron and glia cells transduced with ubiquilin 2 P497S mutant
    Lane 5 : Primary mouse neuron and glia cell cultures transduced with enhanced GFP control
    Lane 6 : HeLa cell lysate
    Lane 7 : 3T3 cell lysate

    Predicted band size: 66 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-UBQLN2 antibody [6H9] (ab190283)
    Immunocytochemistry/ Immunofluorescence - Anti-UBQLN2 antibody [6H9] (ab190283)

    Immunofluorescent analysis of HeLa cells labeling UBQLN2 with ab190283 at 1/1000 dilution. Vimentin is stained in red.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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