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Epigenetics and Nuclear Signaling Chromatin Modifying Enzymes Ubiquitylation

Anti-Ube4a antibody [EPR7331] - BSA and Azide free (ab248191)

Anti-Ube4a antibody [EPR7331] - BSA and Azide free (ab248191)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR7331] to Ube4a - BSA and Azide free
  • Suitable for: ICC, WB
  • Reacts with: Human

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Overview

  • Product name

    Anti-Ube4a antibody [EPR7331] - BSA and Azide free
    See all Ube4a primary antibodies
  • Description

    Rabbit monoclonal [EPR7331] to Ube4a - BSA and Azide free
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    WB
    Human
    See all applications and species data
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    Ab248191 is the carrier-free version of ab128861. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab248191 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.
  • Dissociation constant (KD)

    KD = 1.91 x 10 -11 M
    Learn more about KD
  • Storage buffer

    pH: 7.2
    Constituent: PBS
  • Carrier free

    Yes
  • Concentration information loading...
  • Purity

    Affinity purified
  • Clonality

    Monoclonal
  • Clone number

    EPR7331
  • Isotype

    IgG
  • Research areas

    • Epigenetics and Nuclear Signaling
    • Chromatin Modifying Enzymes
    • Ubiquitylation
    • Cell Biology
    • Proteolysis / Ubiquitin
    • Proteasome / Ubiquitin
    • Ubiquitin E3 Enzymes
    • Other E3 Ligases

Images

  • Western blot - Anti-Ube4a antibody [EPR7331] - BSA and Azide free (ab248191)
    Western blot - Anti-Ube4a antibody [EPR7331] - BSA and Azide free (ab248191)
    All lanes : Anti-Ube4a antibody [EPR7331] (ab128861) at 1/1000 dilution

    Lane 1 : 293T (Human embryonic kidney epithelial cell) cell lysate
    Lane 2 : K562 cell lysate
    Lane 3 : Caco-2 cell lysate
    Lane 4 : HepG2 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP labelled goat anti-rabbit IgG at 1/2000 dilution

    Predicted band size: 123 kDa
    Observed band size: 125 kDa
    why is the actual band size different from the predicted?



    This data was developed using ab128861, the same antibody clone in a different buffer formulation.

  • Immunocytochemistry - Anti-Ube4a antibody [EPR7331] - BSA and Azide free (ab248191)
    Immunocytochemistry - Anti-Ube4a antibody [EPR7331] - BSA and Azide free (ab248191)
    This data was developed using ab128861, the same antibody clone in a different buffer formulation.ab128861, at 1/100 dilution, staining Ube4a in Caco 2 cells by Immunofluorescence.
  • OI-RD Scanning - Anti-Ube4a antibody [EPR7331] - BSA and Azide free (ab248191)
    OI-RD Scanning - Anti-Ube4a antibody [EPR7331] - BSA and Azide free (ab248191)
    This data was developed using ab128861, the same antibody clone in a different buffer formulation.Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD
  • Anti-Ube4a antibody [EPR7331] - BSA and Azide free (ab248191)
    Anti-Ube4a antibody [EPR7331] - BSA and Azide free (ab248191)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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