Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-UBE2M/UBC12 antibody [EPR5333] - BSA and Azide free (ab236056)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR5333] to UBE2M/UBC12 - BSA and Azide free
  • Suitable for: Flow Cyt (Intra), IHC-P, WB, ICC/IF
  • Reacts with: Human

Overview

  • Product name

    Anti-UBE2M/UBC12 antibody [EPR5333] - BSA and Azide free
    See all UBE2M/UBC12 primary antibodies

  • Description

    Rabbit monoclonal [EPR5333] to UBE2M/UBC12 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: Flow Cyt (Intra), IHC-P, WB, ICC/IFmore details

  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: 293T and Ramos cell lysates. IHC-P: Human colonic adenocarcinoma and lung carcinoma tissues. ICC/IF: Ramos cells.

  • General notes

    ab236056 is the carrier-free version of ab109507.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

Images

  • Immunocytochemistry/ Immunofluorescence - Anti-UBE2M/UBC12 antibody [EPR5333] - BSA and Azide free (ab236056)
    Immunocytochemistry/ Immunofluorescence - Anti-UBE2M/UBC12 antibody [EPR5333] - BSA and Azide free (ab236056)

    Immunocytochemistry/ Immunofluorescence analysis of Ramos (human Burkitt's lymphoma B lymphocyte) cells labeling UBE2M/UBC12 with purified ab109507 at 1/250 dilution (8 µg/mL). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with None. Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/1000 (2 µg/mL) dilution. DAPI (blue) was used as nuclear counterstain. 

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab109507).

  • Flow Cytometry - Anti-UBE2M/UBC12 antibody [EPR5333] - BSA and Azide free (ab236056)
    Flow Cytometry - Anti-UBE2M/UBC12 antibody [EPR5333] - BSA and Azide free (ab236056)
    Overlay histogram showing Ramos cells stained with ab109507 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab109507, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1µg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in Ramos cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab109507).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UBE2M/UBC12 antibody [EPR5333] - BSA and Azide free (ab236056)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UBE2M/UBC12 antibody [EPR5333] - BSA and Azide free (ab236056)

    Immunohistochemical analysis of paraffin-embedded Human lung carcinoma tissue using ab109507 at a dilution of 1/100.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab109507).

    Heat mediated antigen retrieval was performed with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UBE2M/UBC12 antibody [EPR5333] - BSA and Azide free (ab236056)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UBE2M/UBC12 antibody [EPR5333] - BSA and Azide free (ab236056)

    Immunohistochemical analysis of paraffin-embedded human colonic adenocarcinoma tissue using ab109507 at a dilution of 1/100.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab109507).

    Heat mediated antigen retrieval was performed with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Anti-UBE2M/UBC12 antibody [EPR5333] - BSA and Azide free (ab236056)
    Anti-UBE2M/UBC12 antibody [EPR5333] - BSA and Azide free (ab236056)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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