Anti-UBE2C antibody (ab3935)
Key features and details
- Goat polyclonal to UBE2C
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-UBE2C antibody
See all UBE2C primary antibodies -
Description
Goat polyclonal to UBE2C -
Host species
Goat -
Tested Applications & Species
See all applications and species dataApplication Species IP HumanWB Human
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Immunogen
Synthetic peptide: QETYSKQVTSQEP, corresponding to C terminal amino acids 167-179 of Human UBE2C.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% Tris, 0.5% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-UBE2C antibody (ab3935)
ab3935 at a 2.5
µ g/ml concentration staining approximately 20 kDa UBE2C by Western blot (ECL):Lane 1 = pCDNA3 transfected U2OS whole cell lysate (2
ab3935 at a 2.5µg/ml concentration staining approximately 20 kDa UBE2C by Western blot (ECL):µ l).
Lane 2 = pCDNA3 MycUbcH10 transfected whole cell lysate (2µ l).
Lane 3 = Nocodazole arrested HeLa cell whole cell lysate (20µ l).
Lane 4 = Asynchronous HeLa cell whole cell lysate (20µ l).
For a more detailed description, please click the reviews tab at the top of the page and see the review dated 25/02/03.
Lane 1 = pCDNA3 transfected U2OS whole cell lysate (2µl).
Lane 2 = pCDNA3 MycUbcH10 transfected whole cell lysate (2µl).
Lane 3 = Nocodazole arrested HeLa cell whole cell lysate (20µl).
Lane 4 = Asynchronous HeLa cell whole cell lysate (20µl).
For a more detailed descri -
Immunoprecipitation - Anti-UBE2C antibody (ab3935)ab3935 staining approximately 20 kDa human UBE2 after immmunoprecipitation from HeLa whole cell lysate (106 cells/ml) using 40
µ l of ab3935 saturated protein G dynabeads, by Western blot (ECL):Lane 1 = IP from Noc arrested HeLa cell extract (beads only).
Lane 2 = IP from Noc arrested HeLa cell extract (goat whole serum coupled to beads).
Lane 3 = IP from Noc arrested HeLa cell extract (ab3935 coupled to beads)
Lane 4 = Noc arrested HeLa whole cell lysate.For a more detailed description, please click the reviews tab at the top of the page and see the review dated 25/02/03.
