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Anti-UBC4 antibody (ab106314)

Price and availability

264 681 ₸

Availability

Order now and get it on Thursday February 25, 2021

Anti-UBC4 antibody (ab106314)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Rabbit polyclonal to UBC4
  • Suitable for: WB
  • Reacts with: Mouse, Human
  • Isotype: IgG

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Overview

  • Product name

    Anti-UBC4 antibody
    See all UBC4 primary antibodies
  • Description

    Rabbit polyclonal to UBC4
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat, Horse, Chicken, Cow, Dog, Pig, Zebrafish
  • Immunogen

    Synthetic peptide corresponding to Human UBC4 aa 100 to the C-terminus (C terminal) conjugated to keyhole limpet haemocyanin.
    (Peptide available as ab125940)

  • Positive control

    • This antibody gave a positive signal in the following whole cell lysates: HeLa; Jurkat; NIH3T3; HEK293; HepG2; MCF7.
  • General notes

     This product was previously labelled as UBE2D2

     

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituent: PBS

    Batches of this product that have a concentration
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Epigenetics and Nuclear Signaling
    • Ubiquitin & Ubiquitin Like Modifiers
    • E2 Ubiquitin Conjugating Enzymes
    • Cell Biology
    • Proteolysis / Ubiquitin
    • Proteasome / Ubiquitin
    • Ubiquitin E2 Enzymes

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Recombinant Protein

    • Recombinant Human UBC4 protein (ab109971)

Applications

Our Abpromise guarantee covers the use of ab106314 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 16 kDa (predicted molecular weight: 16 kDa).

Target

  • Function

    Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro catalyzes 'Lys-48'-linked polyubiquitination. Mediates the selective degradation of short-lived and abnormal proteins. Functions in the E6/E6-AP-induced ubiquitination of p53/TP53. Mediates ubiquitination of PEX5 and autoubiquitination of STUB1 and TRAF6. Involved in the signal-induced conjugation and subsequent degradation of NFKBIA, FBXW2-mediated GCM1 ubiquitination and degradation, MDM2-dependent degradation of p53/TP53 and the activation of MAVS in the mitochondria by DDX58/RIG-I in response to viral infection. Essential for viral activation of IRF3.
  • Pathway

    Protein modification; protein ubiquitination.
  • Sequence similarities

    Belongs to the ubiquitin-conjugating enzyme family.
  • Target information above from: UniProt accession P62837 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 7322 Human
    • Entrez Gene: 56550 Mouse
    • Entrez Gene: 641452 Rat
    • Omim: 602962 Human
    • SwissProt: P62837 Human
    • SwissProt: P62838 Mouse
    • SwissProt: Q06AA9 Pig
    • SwissProt: P62839 Rat
    • Unigene: 108332 Human
    • Unigene: 180052 Mouse
    • Unigene: 7390 Rat
    see all
  • Alternative names

    • E2(17)KB2 antibody
    • OTTHUMP00000223475 antibody
    • OTTHUMP00000223476 antibody
    • OTTHUMP00000223477 antibody
    • OTTHUMP00000223478 antibody
    • OTTHUMP00000224375 antibody
    • PUBC 1 antibody
    • PUBC1 antibody
    • UB2D2_HUMAN antibody
    • UBC 4 antibody
    • UBC 4/5 antibody
    • UBC4 antibody
    • UBC4/5 antibody
    • UBC4/5 homolog yeast antibody
    • UBCH 5B antibody
    • UBCH5B antibody
    • UBE2D2 antibody
    • Ubiquitin carrier protein antibody
    • Ubiquitin carrier protein D2 antibody
    • Ubiquitin conjugating enzyme E2 17 kDa 2 antibody
    • Ubiquitin conjugating enzyme E2 D2 antibody
    • Ubiquitin conjugating enzyme E2D 2 (homologous to yeast UBC4/5) antibody
    • Ubiquitin conjugating enzyme E2D 2 (UBC4/5 homolog yeast) antibody
    • Ubiquitin conjugating enzyme E2D 2 antibody
    • Ubiquitin protein ligase D2 antibody
    • Ubiquitin-conjugating enzyme E2 D2 antibody
    • Ubiquitin-conjugating enzyme E2(17)KB 2 antibody
    • Ubiquitin-conjugating enzyme E2-17 kDa 2 antibody
    • Ubiquitin-protein ligase D2 antibody
    see all

Images

  • Western blot - Anti-UBC4 antibody (ab106314)
    Western blot - Anti-UBC4 antibody (ab106314)
    All lanes : Anti-UBC4 antibody (ab106314) at 1 µg/ml

    Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
    Lane 3 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
    Lane 4 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate
    Lane 5 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
    Lane 6 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 16 kDa
    Observed band size: 16 kDa
    Additional bands at: 55 kDa, 75 kDa. We are unsure as to the identity of these extra bands.


    Exposure time: 8 minutes

Protocols

  • Western blot protocols

Click here to view the general protocols

Datasheets and documents

    • Datasheet
  • References (0)

    Publishing research using ab106314? Please let us know so that we can cite the reference in this datasheet.

    ab106314 has not yet been referenced specifically in any publications.

    Images

    • Western blot - Anti-UBC4 antibody (ab106314)
      Western blot - Anti-UBC4 antibody (ab106314)
      All lanes : Anti-UBC4 antibody (ab106314) at 1 µg/ml

      Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
      Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
      Lane 3 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
      Lane 4 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate
      Lane 5 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
      Lane 6 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

      Developed using the ECL technique.

      Performed under reducing conditions.

      Predicted band size: 16 kDa
      Observed band size: 16 kDa
      Additional bands at: 55 kDa, 75 kDa. We are unsure as to the identity of these extra bands.


      Exposure time: 8 minutes

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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