Anti-TXNDC5 antibody (ab13820)
Key features and details
- Goat polyclonal to TXNDC5
- Suitable for: IHC-P, WB, Flow Cyt, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-TXNDC5 antibody
See all TXNDC5 primary antibodies -
Description
Goat polyclonal to TXNDC5 -
Host species
Goat -
Specificity
This antibody is expected to be able to recognise both reported human isoforms, as represented by NP_110437; NP_071368. -
Tested applications
Suitable for: IHC-P, WB, Flow Cyt, ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide: SLHRFVLSQAKDEL, corresponding to C terminal amino acids 347-360 of Human TXNDC5.
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General notes
GenBank Accession Number - NP_110437; NP_071368
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.3
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunocytochemistry/ Immunofluorescence - Anti-TXNDC5 antibody (ab13820)
Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation with ab13820 1hr (10 μg/ml) followed by Alexa Fluor 488 secondary antibody (2 μg/ml), showing endoplasmic reticulum staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 μg/ml) followed by Alexa Fluor 488 secondary antibody (2 μg/ml).
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Flow Cytometry - Anti-TXNDC5 antibody (ab13820)Flow cytometric analysis of paraformaldehyde fixed HeLa cells (blue line), permeabilized with 0.5% Triton. Primary incubation with ab13820 for 1hr (10 μg/ml) followed by Alexa Fluor 488 secondary antibody (1 μg/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
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Immunocytochemistry/ Immunofluorescence - Anti-TXNDC5 antibody (ab13820)Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation with ab13820 1hr (10 μg/ml) followed by Alexa Fluor 488 secondary antibody (2 μg/ml), showing endoplasmic reticulum staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 μg/ml) followed by Alexa Fluor 488 secondary antibody (2 μg/ml).
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Western blot - Anti-TXNDC5 antibody (ab13820)All lanes : Anti-TXNDC5 antibody (ab13820) at 0.1 µg/ml
Lane 1 : HEK-293 cell lysate
Lane 2 : A549 cell lysate
Lane 3 : HeLa cell lysate
Lane 4 : HepG2 cell lysate
Lysates/proteins at 35 µg per lane.
Predicted band size: 50 kDa
