Anti-TUBA1B antibody (ab151424)
Key features and details
- Rabbit polyclonal to TUBA1B
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-TUBA1B antibody
See all TUBA1B primary antibodies -
Description
Rabbit polyclonal to TUBA1B -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Recombinant fragment, corresponding to a region within amino acids 200-440 of Human TUBA1B.
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Positive control
- Jurkat, Raji, 293T, A431, HeLa, HepG2, H1299, HCT116, MCF7, NT2D1, PC3, U-87 MG, NIH 3T3 and PC12 whole cell lysates; HeLa cells; Mouse U251 xenograft tissue.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 78% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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All lanes : Anti-TUBA1B antibody (ab151424) at 1/10000 dilution
Lane 1 : A431 whole cell lysate
Lane 2 : Jurkat whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 50 kDa
10% SDS-PAGE. -
All lanes : Anti-TUBA1B antibody (ab151424) at 1/10000 dilution
Lane 1 : Jurkat whole cell lysate
Lane 2 : Raji whole cell lysate
Lane 3 : 293T whole cell lysate
Lane 4 : A431 whole cell lysate
Lane 5 : HeLa whole cell lysate
Lane 6 : HepG2 whole cell lysate
Lane 7 : H1299 whole cell lysate
Lane 8 : HCT116 whole cell lysate
Lane 9 : MCF7 whole cell lysate
Lane 10 : NT2D1 whole cell lysate
Lane 11 : PC3 whole cell lysate
Lane 12 : U-87 MG whole cell lysate
Predicted band size: 50 kDa
7.5% SDS-PAGE. -
All lanes : Anti-TUBA1B antibody (ab151424) at 1/10000 dilution
Lane 1 : 293T whole cell lysate at 20 µg
Lane 2 : 293T whole cell lysate at 10 µg
Lane 3 : 293T whole cell lysate at 5 µg
Lane 4 : 293T whole cell lysate at 1 µg
Predicted band size: 50 kDa
7.5% SDS-PAGE. -
All lanes : Anti-TUBA1B antibody (ab151424) at 1/10000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : HeLa cell lysate, cytosol fraction
Lane 3 : HeLa cell lysate, nucleus fraction
Lysates/proteins at 30 µg per lane.
Predicted band size: 50 kDa
7.5% SDS-PAGE. -
All lanes : Anti-TUBA1B antibody (ab151424) at 1/10000 dilution
Lane 1 : 293T whole cell lysate
Lane 2 : NIH 3T3 whole cell lysate
Lane 3 : PC12 whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 50 kDa
7.5% SDS-PAGE. -
Confocal Immunofluorescent analysis of methanol-fixed HeLa cells labeling TUBA1B with ab151424 at 1/500 dilution (green).
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Immunohistochemical analysis of paraffin-embedded Mouse U251 xenograft tissue labeling TUBA1B with ab151424 at 1/500 dilution.