Antibodies, Proteins, Kits and Reagents for Life Science

291 484 ₸ Product size

100 µl 291 484 ₸

Order now and get it on Wednesday March 10, 2021

Anti-TTC19 antibody [EPR13184] - C-terminal (ab181069)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR13184] to TTC19 - C-terminal
Suitable for: WB, ICC/IF, IP
Reacts with: Human

Product name

Anti-TTC19 antibody [EPR13184] - C-terminal
See all TTC19 primary antibodies
Description

Rabbit monoclonal [EPR13184] to TTC19 - C-terminal
Host species

Rabbit

Tested Applications & Species

Application	Species
ICC/IF	Human
IP	Human
WB	Human

See all applications and species data

Immunogen

This information is proprietary to Abcam and/or its suppliers.
Positive control
- HT-1080 cell and Human fetal muscle, fetal heart and ovarian cancer lysates. HT-1080 cells.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR13184
Isotype

IgG
Research areas

Western blot - Anti-TTC19 antibody [EPR13184] - C-terminal (ab181069)

All lanes : Anti-TTC19 antibody [EPR13184] - C-terminal (ab181069) at 1/2000 dilution

Lane 1 : Human fetal muscle tissue lysate
Lane 2 : Human ovarian cancer tissue lysate
Lane 3 : Human fetal heart tissue lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab136636) at 1/1000 dilution

Predicted band size: 42 kDa
Observed band size: 35 kDa
why is the actual band size different from the predicted?
Western blot - Anti-TTC19 antibody [EPR13184] - C-terminal (ab181069)

Anti-TTC19 antibody [EPR13184] - C-terminal (ab181069) at 1/10000 dilution + HT-1080 cell lysate at 20 µg

Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab136636) at 1/500 dilution

Predicted band size: 42 kDa
Observed band size: 35 kDa why is the actual band size different from the predicted?
Immunocytochemistry/ Immunofluorescence - Anti-TTC19 antibody [EPR13184] - C-terminal (ab181069)

Immunofluorescence analysis of HT-1080 cells, staining TTC19 (green) with ab181069. Goat anti rabbit IgG (Alexa Fluor®488) was used as a secondary antibody at 1/200 dilution. Counterstained with DAPI (blue)
Immunoprecipitation - Anti-TTC19 antibody [EPR13184] - C-terminal (ab181069)

Western blot analysis on immunoprecipitation pellet from HT-1080, labeling TTC19 immunoprecipitated using ab181069 at 1/70 dilution and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.
Anti-TTC19 antibody [EPR13184] - C-terminal (ab181069)

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