Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-TTC19 antibody [EPR13184] - C-terminal (ab181069)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR13184] to TTC19 - C-terminal
  • Suitable for: WB, ICC/IF, IP
  • Reacts with: Human

Overview

  • Product name

    Anti-TTC19 antibody [EPR13184] - C-terminal
    See all TTC19 primary antibodies

  • Description

    Rabbit monoclonal [EPR13184] to TTC19 - C-terminal

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    IP
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • HT-1080 cell and Human fetal muscle, fetal heart and ovarian cancer lysates. HT-1080 cells.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR13184

  • Isotype

    IgG

  • Research areas

Images

  • Western blot - Anti-TTC19 antibody [EPR13184] - C-terminal (ab181069)
    Western blot - Anti-TTC19 antibody [EPR13184] - C-terminal (ab181069)
    All lanes : Anti-TTC19 antibody [EPR13184] - C-terminal (ab181069) at 1/2000 dilution

    Lane 1 : Human fetal muscle tissue lysate
    Lane 2 : Human ovarian cancer tissue lysate
    Lane 3 : Human fetal heart tissue lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab136636) at 1/1000 dilution

    Predicted band size: 42 kDa
    Observed band size: 35 kDa
    why is the actual band size different from the predicted?

  • Western blot - Anti-TTC19 antibody [EPR13184] - C-terminal (ab181069)
    Western blot - Anti-TTC19 antibody [EPR13184] - C-terminal (ab181069)
    Anti-TTC19 antibody [EPR13184] - C-terminal (ab181069) at 1/10000 dilution + HT-1080 cell lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab136636) at 1/500 dilution

    Predicted band size: 42 kDa
    Observed band size: 35 kDa why is the actual band size different from the predicted?

  • Immunocytochemistry/ Immunofluorescence - Anti-TTC19 antibody [EPR13184] - C-terminal (ab181069)
    Immunocytochemistry/ Immunofluorescence - Anti-TTC19 antibody [EPR13184] - C-terminal (ab181069)

    Immunofluorescence analysis of HT-1080 cells, staining TTC19 (green) with ab181069. Goat anti rabbit IgG (Alexa Fluor®488) was used as a secondary antibody at 1/200 dilution. Counterstained with DAPI (blue)

  • Immunoprecipitation - Anti-TTC19 antibody [EPR13184] - C-terminal (ab181069)
    Immunoprecipitation - Anti-TTC19 antibody [EPR13184] - C-terminal (ab181069)

    Western blot analysis on immunoprecipitation pellet from HT-1080, labeling TTC19 immunoprecipitated using ab181069 at 1/70 dilution and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.

  • Anti-TTC19 antibody [EPR13184] - C-terminal (ab181069)
    Anti-TTC19 antibody [EPR13184] - C-terminal (ab181069)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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