Anti-TRIF antibody (ab13810)
Key features and details
- Rabbit polyclonal to TRIF
- Suitable for: WB, ICC/IF, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-TRIF antibody
See all TRIF primary antibodies -
Description
Rabbit polyclonal to TRIF -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IF, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Synthetic peptide, corresponding to amino acids 143-158 of Mouse TRIF.
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Positive control
- WB: Mouse spleen, intestine, liver and hepatocytes lysates; Raji and RAW 264.7 cell lysates. IHC-P: Human colon tissue. ICC/IF: A431 cells.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituents: PBS, 0.05% BSA -
Concentration information loading... -
Purity
Whole antiserum -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-TRIF antibody (ab13810)Anti-TRIF antibody (ab13810) at 1/1000 dilution + Mouse spleen lysate
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRIF antibody (ab13810)ab13810 (2µg/ml) staining TRIF in human colon using an automated system (DAKO Autostainer Plus). Using this protocol there is strong staining of mucosal cell cytoplasm.
Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer citrate pH6.1 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.
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Western blot - Anti-TRIF antibody (ab13810)All lanes : Anti-TRIF antibody (ab13810) at 1/1000 dilution
Lane 1 : Mouse spleen lysate with immunizing peptide
Lane 2 : Mouse spleen lysate
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Western blot - Anti-TRIF antibody (ab13810)All lanes : Anti-TRIF antibody (ab13810) at 1/1000 dilution
Lane 1 : Mouse intestine lysate
Lane 2 : Mouse spleen lysate
Lane 3 : RAW 264.7 (mouse macrophage cell line transformed with Abelson murine leukemia virus) cell lysate
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Western blot - Anti-TRIF antibody (ab13810)Anti-TRIF antibody (ab13810) at 1/100 dilution + Raji (human Burkitt's lymphoma cell line) cell lysate
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Immunocytochemistry/ Immunofluorescence - Anti-TRIF antibody (ab13810)A431 (human epidermoid carcinoma cell line) cells stained for TRIF (green) using ab13810 at 1/10 dilution in ICC/IF with a Dylight 488 secondary antibody. Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).
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Western blot - Anti-TRIF antibody (ab13810) This image is courtesy of an anonymous Abreview.All lanes : Anti-TRIF antibody (ab13810) at 1/1000 dilution
Lane 1 : Mouse liver lysate
Lane 2 : Mouse hepatocytes lysate
Secondary
All lanes : Goat anti-rabbit HRP at 1/10000 dilution
