Anti-TRAP220/MED1 (phospho T1457) antibody [EPR13269] - BSA and Azide free (ab250350)
![Anti-TRAP220/MED1 (phospho T1457) antibody [EPR13269] - BSA and Azide free (ab250350)](https://www.abcam.com/ps/products/250/ab250350/Images/ab250350-408418-anti-trap220-med1-phospho-t1457-antibody-epr13269-bsa-and-azide-free-western-blot.jpg "Anti-TRAP220/MED1 (phospho T1457) antibody [EPR13269] - BSA and Azide free (ab250350)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR13269] to TRAP220/MED1 (phospho T1457) - BSA and Azide free
- Suitable for: IHC-P, Dot blot, WB
- Reacts with: Mouse, Rat, Human
Overview
-
Product name
Anti-TRAP220/MED1 (phospho T1457) antibody [EPR13269] - BSA and Azide free
See all TRAP220/MED1 primary antibodies -
Description
Rabbit monoclonal [EPR13269] to TRAP220/MED1 (phospho T1457) - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, Dot blot, WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
-
General notes
Ab250350 is the carrier-free version of ab181103. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab250350 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Affinity purified -
Clonality
Monoclonal -
Clone number
EPR13269 -
Isotype
IgG -
Research areas
Images
-
Western blot - Anti-TRAP220/MED1 (phospho T1457) antibody [EPR13269] - BSA and Azide free (ab250350)All lanes : Anti-TRAP220/MED1 (phospho T1457) antibody [EPR13269] (ab181103) at 1/10000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : HeLa cell lysate treated with Lambda Phosphatase
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 168 kDaThis data was developed using ab181103, the same antibody clone in a different buffer formulation.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRAP220/MED1 (phospho T1457) antibody [EPR13269] - BSA and Azide free (ab250350)](https://www.abcam.com/ps/products/250/ab250350/Images/ab250350-3-anti-trap220-med1-phospho-t1457-antibody-epr13269-bsa-and-azide-free-immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRAP220/MED1 (phospho T1457) antibody [EPR13269] - BSA and Azide free (ab250350)This data was developed using ab181103, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded sections of Human thyroid gland carcinoma tissue labeling TRAP220/MED1 (phospho T1457) using ab181103 at a 1/250 dilution, followed by a HRP Polymer for Rabbit IgG secondary antibody and counterstained with Hematoxylin. Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol. -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRAP220/MED1 (phospho T1457) antibody [EPR13269] - BSA and Azide free (ab250350)](https://www.abcam.com/ps/products/250/ab250350/Images/ab250350-2-anti-trap220-med1-phospho-t1457-antibody-epr13269-bsa-and-azide-free-immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRAP220/MED1 (phospho T1457) antibody [EPR13269] - BSA and Azide free (ab250350)This data was developed using ab181103, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded sections of Human pancreas tissue labeling TRAP220/MED1 (phospho T1457) using ab181103 at a 1/250 dilution, followed by a HRP Polymer for Rabbit IgG secondary antibody and counterstained with Hematoxylin. Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol. -
![Dot Blot - Anti-TRAP220/MED1 (phospho T1457) antibody [EPR13269] - BSA and Azide free (ab250350)](https://www.abcam.com/ps/products/250/ab250350/Images/ab250350-1-anti-trap220-med1-phospho-t1457-antibody-epr13269-bsa-and-azide-free-dot-blot.jpg)
Dot Blot - Anti-TRAP220/MED1 (phospho T1457) antibody [EPR13269] - BSA and Azide free (ab250350)This data was developed using ab181103, the same antibody clone in a different buffer formulation.Dot blot analysis of TRAP220/MED1 (pT1457) peptide (Lane 1), TRAP220/MED1 non-phospho peptide (Lane 2) labelling TRAP220/MED1 (pT1457) with ab181103 at a dilution of 1/1000. A Peroxidase-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody at a dilution of 1/2500. Blocking and dilution buffer: 5% NFDM/TBST. Exposure time: 10 seconds. -
![Anti-TRAP220/MED1 (phospho T1457) antibody [EPR13269] - BSA and Azide free (ab250350)](https://www.abcam.com/ps/products/250/ab250350/Images/ab250350-5-benefits-of-recombinant-antibodies.png)
Anti-TRAP220/MED1 (phospho T1457) antibody [EPR13269] - BSA and Azide free (ab250350)