Anti-Transferrin Receptor antibody [H68.4] (ab269513)
![Anti-Transferrin Receptor antibody [H68.4] (ab269513)](https://www.abcam.com/ps/products/269/ab269513/Images/ab269513-392680-anti-transferrin-receptor-antibody-h684-western-blot-hela.jpg "Anti-Transferrin Receptor antibody [H68.4] (ab269513)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Mouse monoclonal [H68.4] to Transferrin Receptor
- Suitable for: WB, ICC/IF, IHC-P
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-Transferrin Receptor antibody [H68.4]
See all Transferrin Receptor primary antibodies -
Description
Mouse monoclonal [H68.4] to Transferrin Receptor -
Host species
Mouse -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF MouseHumanIHC-P HumanWB MouseRatHuman
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Immunogen
Recombinant fragment within Human Transferrin Receptor. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
Database link: P02786 -
Positive control
- WB: HeLa and K562 whole cell lysate; Human, mouse and rat placenta lysate. IHC-P: Human placenta and cervical carcinoma tissue. ICC/IF: K-562 and RAW 264.7 cells.
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General notes
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 0.05% BSA, 40% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
H68.4 -
Isotype
IgG1 -
Research areas
Images
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Western blot - Anti-Transferrin Receptor antibody [H68.4] (ab269513)All lanes : Anti-Transferrin Receptor antibody [H68.4] (ab269513) at 1/5000 dilution
Lane 1 : HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate
Lane 2 : K562 (human chronic myelogenous leukemia lymphoblast), whole cell lysate
Lane 3 : Human placenta tissue lysate
Lane 4 : Mouse placenta tissue lysate
Lane 5 : Rat placenta tissue lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/10000 dilution
Predicted band size: 84 kDa
Observed band size: 90 kDa why is the actual band size different from the predicted?Transferrin receptor can undergo glycosylation as shown in lane 4 and 5 (PMID: 30854239).
Blocking/Diliution buffer: 5% NFDM/TBST.
Exposure times: Lanes 1/3-5: 114 seconds; Lane 2: 3 minutes.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Transferrin Receptor antibody [H68.4] (ab269513)](https://www.abcam.com/ps/products/269/ab269513/Images/ab269513-1-anti-transferrin-receptor-antibody-h684-immunohistochemistry-human-placenta.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Transferrin Receptor antibody [H68.4] (ab269513)Immunohistochemical analysis of paraffin-embedded human placenta tissue labeling Transferrin Receptor with ab269513 at 1/2000 dilution followed by ready to use Goat Anti-mouse IgG H&L (HRP polymer) (ab214879). Positive staining on human placenta. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is ready to use Goat Anti-mouse IgG H&L (HRP polymer) (ab214879).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 10 mins.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Transferrin Receptor antibody [H68.4] (ab269513)](https://www.abcam.com/ps/products/269/ab269513/Images/ab269513-2-anti-transferrin-receptor-antibody-h684-immunohistochemistry-human-cervical-carcinoma.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Transferrin Receptor antibody [H68.4] (ab269513)Immunohistochemical analysis of paraffin-embedded human cervical carcinoma tissue labeling Transferrin Receptor with ab269513 at 1/2000 dilution followed by ready to use Goat Anti-mouse IgG H&L (HRP polymer) (ab214879). Positive staining on human cervical carcinoma. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is ready to use Goat Anti-mouse IgG H&L (HRP polymer) (ab214879).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 10 mins.
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![Immunocytochemistry/ Immunofluorescence - Anti-Transferrin Receptor antibody [H68.4] (ab269513)](https://www.abcam.com/ps/products/269/ab269513/Images/ab269513-3-anti-transferrin-receptor-antibody-h684-immunocytochemistry-k562.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Transferrin Receptor antibody [H68.4] (ab269513)Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized K-562 cells labelling Transferrin Receptor with ab269513 at 1/25 dilution, followed by ab150113 Goat Anti-mouse IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green). Confocal image showing cytoplasmic staining in K-562 cell line. ab179513 Anti-beta Tubulin rabbit monoclonal antibody was used to counterstain tubulin at 1/200 dilution, followed by ab150080 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) at 1/500 dilution (Red). The nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Secondary antibody is ab150113 Goat Anti-mouse IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.
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![Immunocytochemistry/ Immunofluorescence - Anti-Transferrin Receptor antibody [H68.4] (ab269513)](https://www.abcam.com/ps/products/269/ab269513/Images/ab269513-4-anti-transferrin-receptor-antibody-h684-immunocytochemistry-raw-2647.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Transferrin Receptor antibody [H68.4] (ab269513)Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized RAW 264.7 cells labelling Transferrin Receptor with ab269513 at 1/25 dilution, followed by ab150113 Goat Anti-mouse IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green). Confocal image showing cytoplasmic staining in RAW 264.7 cell line. ab179513 Anti-beta Tubulin rabbit monoclonal antibody was used to counterstain tubulin at 1/200 dilution, followed by ab150080 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) at 1/500 dilution (Red). The nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Secondary antibody is ab150113 Goat Anti-mouse IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.
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![Anti-Transferrin Receptor antibody [H68.4] (ab269513)](https://www.abcam.com/ps/products/269/ab269513/Images/ab269513-6-benefits-of-recombinant-antibodies.png)
Anti-Transferrin Receptor antibody [H68.4] (ab269513)