Anti-TRAFD1 antibody - N-terminal (ab201308)
Key features and details
- Rabbit polyclonal to TRAFD1 - N-terminal
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-TRAFD1 antibody - N-terminal
See all TRAFD1 primary antibodies -
Description
Rabbit polyclonal to TRAFD1 - N-terminal -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IP HumanWB Human -
Immunogen
Synthetic peptide within Human TRAFD1 aa 1-50 (N terminal). The exact sequence is proprietary. NP_001137378.1
Database link: O14545 -
Positive control
- WB: HeLa, 293T and Jurkat whole cell lysates. IP: 293T cell lysate.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH 7 to 8 -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Detection of FLN29 by immunoprecipitation of whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T using NETN lysis buffer. Lane 1: Anti-FLN29 antibody (ab201308) at 6 µg/mg lysate, Lane 2: Anti-FLN29 antibody (ab201308) at 6 µg/mg lysate, Lane 3: Control IgG was used as a negative control. Detection: Chemiluminescence with an exposure time of 30 seconds.
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All lanes : Anti-TRAFD1 antibody - N-terminal (ab201308) at 0.1 µg/ml
Lane 1 : HeLa whole cell lysate
Lane 2 : 293T whole cell lysate
Lane 3 : Jurkat whole cell lysate
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Predicted band size: 65 kDa
Exposure time: 30 seconds
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All lanes : Anti-TRAFD1 antibody - N-terminal (ab201308) at 0.4 µg/ml
Lane 1 : Immunoprecipitation using ab201308 at 6 µg/mg lysate (1 mg/IP; 20% of IP loaded/lane) from 293T cells prepared using NETN lysis buffer
Lane 2 : Immunoprecipitation using control IgG
Developed using the ECL technique.
Predicted band size: 65 kDa
Exposure time: 30 seconds