Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-TRAF1 antibody [1F3] - BSA and Azide free (ab255878)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rat monoclonal [1F3] to TRAF1 - BSA and Azide free
  • Suitable for: WB
  • Reacts with: Human

Overview

  • Product name

    Anti-TRAF1 antibody [1F3] - BSA and Azide free
    See all TRAF1 primary antibodies

  • Description

    Rat monoclonal [1F3] to TRAF1 - BSA and Azide free

  • Host species

    Rat

  • Tested Applications & Species

    Application Species
    WB
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is considered to be commercially sensitive.

  • Positive control

    • WB: NK-92, Daudi and Raji whole cell lysates; THP-1 treated with 5ng/ml TNF-alpha for 6h, whole cell lysate.

  • General notes

    This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

    ab255878 is the carrier-free version of ab255878. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

Properties

Images

  • Western blot - Anti-TRAF1 antibody [1F3] - BSA and Azide free (ab255878)
    Western blot - Anti-TRAF1 antibody [1F3] - BSA and Azide free (ab255878)
    All lanes : Anti-TRAF1 antibody [1F3] (ab252875) at 1/1000 dilution

    Lane 1 : THP-1 (human monocytic leukemia monocyte), whole cell lysate
    Lane 2 : THP-1 treated with 5ng/ml TNF-alpha for 6h, whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rat IgG H&L (HRP) (ab205720) at 1/5000 dilution

    Predicted band size: 46 kDa
    Observed band size: 50 kDa
    why is the actual band size different from the predicted?


    Exposure time: 3 minutes


    Blocking/Dilution buffer: 5% NFDM/TBST.

    This image was produced using the same antibody clone in a different buffer formulation containing PBS, glycerol, BSA and sodium azide (ab252875).

  • Western blot - Anti-TRAF1 antibody [1F3] - BSA and Azide free (ab255878)
    Western blot - Anti-TRAF1 antibody [1F3] - BSA and Azide free (ab255878)
    All lanes : Anti-TRAF1 antibody [1F3] (ab252875) at 1/1000 dilution

    Lane 1 : NK-92 (human malignant non-Hodgkin's lymphoma natural killer cell), whole cell lysate
    Lane 2 : Daudi (human Burkitt's lymphoma lymphoblast), whole cell lysate
    Lane 3 : Raji (human Burkitt's lymphoma lymphoblast), whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rat IgG H&L (HRP) (ab205720) at 1/5000 dilution

    Predicted band size: 46 kDa
    Observed band size: 50 kDa why is the actual band size different from the predicted?



    The bands observed in lane 1 is consistent with what has been described in the literature (PMID: 11181075).

    Blocking/Dilution buffer: 5% NFDM/TBST.

    Exposure times: Lane 1-2: 3 mins; Lane 3: 37 secs.

    This image was produced using the same antibody clone in a different buffer formulation containing PBS, glycerol, BSA and sodium azide (ab252875).

  • Anti-TRAF1 antibody [1F3] - BSA and Azide free (ab255878)
    Anti-TRAF1 antibody [1F3] - BSA and Azide free (ab255878)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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