Anti-TRAF1 antibody [1F3] - BSA and Azide free (ab255878)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rat monoclonal [1F3] to TRAF1 - BSA and Azide free
- Suitable for: WB
- Reacts with: Human
Overview
-
Product name
Anti-TRAF1 antibody [1F3] - BSA and Azide free
See all TRAF1 primary antibodies -
Description
Rat monoclonal [1F3] to TRAF1 - BSA and Azide free -
Host species
Rat -
Tested Applications & Species
See all applications and species dataApplication Species WB Human -
Immunogen
Recombinant fragment. This information is considered to be commercially sensitive.
-
Positive control
- WB: NK-92, Daudi and Raji whole cell lysates; THP-1 treated with 5ng/ml TNF-alpha for 6h, whole cell lysate.
-
General notes
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
ab255878 is the carrier-free version of ab255878. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading...
-
Purity
Ion Exchange Chromatography -
Clonality
Monoclonal -
Clone number
1F3 -
Isotype
IgG2a -
Light chain type
kappa -
Research areas
Images
-
All lanes : Anti-TRAF1 antibody [1F3] (ab252875) at 1/1000 dilution
Lane 1 : THP-1 (human monocytic leukemia monocyte), whole cell lysate
Lane 2 : THP-1 treated with 5ng/ml TNF-alpha for 6h, whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rat IgG H&L (HRP) (ab205720) at 1/5000 dilution
Predicted band size: 46 kDa
Observed band size: 50 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutesBlocking/Dilution buffer: 5% NFDM/TBST.
This image was produced using the same antibody clone in a different buffer formulation containing PBS, glycerol, BSA and sodium azide (ab252875).
-
All lanes : Anti-TRAF1 antibody [1F3] (ab252875) at 1/1000 dilution
Lane 1 : NK-92 (human malignant non-Hodgkin's lymphoma natural killer cell), whole cell lysate
Lane 2 : Daudi (human Burkitt's lymphoma lymphoblast), whole cell lysate
Lane 3 : Raji (human Burkitt's lymphoma lymphoblast), whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rat IgG H&L (HRP) (ab205720) at 1/5000 dilution
Predicted band size: 46 kDa
Observed band size: 50 kDa why is the actual band size different from the predicted?The bands observed in lane 1 is consistent with what has been described in the literature (PMID: 11181075).
Blocking/Dilution buffer: 5% NFDM/TBST.
Exposure times: Lane 1-2: 3 mins; Lane 3: 37 secs.
This image was produced using the same antibody clone in a different buffer formulation containing PBS, glycerol, BSA and sodium azide (ab252875).
-