Anti-TRADD antibody [37/TRADD] (ab280374)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Mouse monoclonal [37/TRADD] to TRADD
- Suitable for: WB
- Reacts with: Rat, Human
Overview
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Product name
Anti-TRADD antibody [37/TRADD]
See all TRADD primary antibodies -
Description
Mouse monoclonal [37/TRADD] to TRADD -
Host species
Mouse -
Tested applications
Suitable for: WBmore details
Unsuitable for: ICC or IHC-P -
Species reactivity
Reacts with: Rat, Human -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: HeLa, Ramos, and K-562 whole cell lysates; Human liver, brain and heart tissue lysates; Rat kidney tissue lysate.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
37/TRADD -
Isotype
IgG1
Images
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All lanes : Anti-TRADD antibody [37/TRADD] (ab280374) at 1/1000 dilution
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma), whole cell lysate
Lane 2 : Human liver tissue lysate
Lane 3 : Ramos (Human Burkitt’s lymphoma B lymphocyte), whole cell lysate
Lane 4 : K-562 (human chronic myelogenous leukemia lymphoblast), whole cell lysate
Lane 5 : Rat kidney tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/10000 dilution
Predicted band size: 34 kDa
Observed band size: 34 kDaBlocking buffer and Diluting buffer and concentration: 5% NFDM/TBST.
Exposure time 103 seconds.
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All lanes : Anti-TRADD antibody [37/TRADD] (ab280374) at 1/1000 dilution
Lane 1 : Human brain tissue lysate
Lane 2 : Human heart tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/10000 dilution
Predicted band size: 34 kDa
Observed band size: 34 kDaBlocking buffer and Diluting buffer and concentration: 5% NFDM/TBST.
Exposure time 3 minutes.
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