Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Topoisomerase II alpha (phospho T1343) antibody [EP1054Y] - BSA and Azide free (ab247333)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EP1054Y] to Topoisomerase II alpha (phospho T1343) - BSA and Azide free
  • Suitable for: IHC-P, WB, ICC/IF
  • Reacts with: Human

Overview

  • Product name

    Anti-Topoisomerase II alpha (phospho T1343) antibody [EP1054Y] - BSA and Azide free
    See all Topoisomerase II alpha primary antibodies

  • Description

    Rabbit monoclonal [EP1054Y] to Topoisomerase II alpha (phospho T1343) - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IHC-P, WB, ICC/IFmore details
    Unsuitable for: Flow Cyt or IP

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • ICC/IF: HeLa cells

  • General notes

    ab247333 is the carrier-free version of ab52853.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Properties

Images

  • Western blot - Anti-Topoisomerase II alpha (phospho T1343) antibody [EP1054Y] - BSA and Azide free (ab247333)
    Western blot - Anti-Topoisomerase II alpha (phospho T1343) antibody [EP1054Y] - BSA and Azide free (ab247333)
    All lanes : Anti-Topoisomerase II alpha (phospho T1343) antibody [EP1054Y] (ab52853) at 1/1000 dilution

    Lane 1 : HeLa cell lysate treated with AP
    Lane 2 : HeLa cell lysate untreated

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : goat anti-rabbit HRP at 1/2000 dilution

    Predicted band size: 174 kDa
    Observed band size: 174 kDa



    This data was developed using ab52853, the same antibody clone in a different buffer formulation.

  • Immunocytochemistry - Anti-Topoisomerase II alpha (phospho T1343) antibody [EP1054Y] - BSA and Azide free (ab247333)
    Immunocytochemistry - Anti-Topoisomerase II alpha (phospho T1343) antibody [EP1054Y] - BSA and Azide free (ab247333)
    This data was developed using the same antibody clone in a different buffer formulation (ab52853).
    Immunocytochemistry analysis of HeLa (human cervix adenocarcinoma epithelial cell) labeling Topoisomerase II alpha with purified ab52853 at 1/50 dilution (4 µg/ml). Cells were fixed with 4% Paraformaldehyde and permeabilised with 0.1% tritonX-100. Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/1000 (2 µg/ml) was used as the secondary antibody. ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.14 µg/ml) was used as counterstain. Nuclei were stained blue with DAPI.
    Negative control: PBS instead of the primary antibody.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Topoisomerase II alpha (phospho T1343) antibody [EP1054Y] - BSA and Azide free (ab247333)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Topoisomerase II alpha (phospho T1343) antibody [EP1054Y] - BSA and Azide free (ab247333)
    This data was developed using ab52853, the same antibody clone in a different buffer formulation.ab52853 at 1/100 dilution staining Topoisomerase II alpha in paraffin embedded human cervical carcinoma tissue by Immunohistochemistry. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • Anti-Topoisomerase II alpha (phospho T1343) antibody [EP1054Y] - BSA and Azide free (ab247333)
    Anti-Topoisomerase II alpha (phospho T1343) antibody [EP1054Y] - BSA and Azide free (ab247333)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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