Anti-TNF alpha antibody [TNFA/1172] (ab220210)
![Anti-TNF alpha antibody [TNFA/1172] (ab220210)](https://www.abcam.com/ps/products/220/ab220210/Images/ab220210-277566-anti-tnf-alpha-antibody-tnfa-1172-immunohistochemistry.jpg "Anti-TNF alpha antibody [TNFA/1172] (ab220210)")
Key features and details
- Mouse monoclonal [TNFA/1172] to TNF alpha
- Suitable for: IHC-P, ICC
- Reacts with: Rat, Human
- Isotype: IgM
Overview
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Product name
Anti-TNF alpha antibody [TNFA/1172]
See all TNF alpha primary antibodies -
Description
Mouse monoclonal [TNFA/1172] to TNF alpha -
Host species
Mouse -
Tested Applications & Species
See all applications and species dataApplication Species ICC HumanIHC-P RatHuman
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Immunogen
Recombinant full length protein corresponding to Human TNF alpha.
Database link: P01375 -
Positive control
- IHC-P: Human Erdheim Chester disease, rat stomach and rat pancreas tissues; ICC: HepG2 cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.05% Sodium azide
Constituents: 0.05% BSA, PBS -
Concentration information loading... -
Purity
Protein A/G purified -
Clonality
Monoclonal -
Clone number
TNFA/1172 -
Isotype
IgM -
Light chain type
kappa -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TNF alpha antibody [TNFA/1172] (ab220210)
Immunohistochemical analysis of formalin-fixed and paraffin-embedded rat pancreas tissue labeling TNF alpha with ab220210 at 4 μg/mL.
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![Immunocytochemistry - Anti-TNF alpha antibody [TNFA/1172] (ab220210)](https://www.abcam.com/ps/products/220/ab220210/Images/ab220210-5-anti-tnf-alpha-antibody-tnfa-1172-immunocytochemistry-hepg2-human.jpg)
Immunocytochemistry - Anti-TNF alpha antibody [TNFA/1172] (ab220210)Immunofluorescence analysis of PFA-fixed HepG2 (human liver hepatocellular carcinoma cell line) cells labeling TNF alpha with ab220210 at 2 µg/mL followed by Goat anti-Mouse IgG-CF488 (green). The nuclear counterstain is Redot (red). -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TNF alpha antibody [TNFA/1172] (ab220210)](https://www.abcam.com/ps/products/220/ab220210/Images/ab220210-277565-anti-tnf-alpha-antibody-tnfa-1172-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TNF alpha antibody [TNFA/1172] (ab220210)Immunohistochemical analysis of formalin-fixed and paraffin-embedded rat stomach tissue labeling TNF alpha with ab220210 at 4 μg/mL.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TNF alpha antibody [TNFA/1172] (ab220210)](https://www.abcam.com/ps/products/220/ab220210/Images/ab220210-277564-anti-tnf-alpha-antibody-tnfa-1172-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TNF alpha antibody [TNFA/1172] (ab220210)Immunohistochemical analysis of formalin-fixed and paraffin-embedded Human Erdheim Chester disease tissue labeling TNF alpha with ab220210 at 4 μg/mL.
