Anti-TNF alpha antibody (ab9635)
Key features and details
- Rabbit polyclonal to TNF alpha
- Suitable for: WB, Sandwich ELISA
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-TNF alpha antibody
See all TNF alpha primary antibodies -
Description
Rabbit polyclonal to TNF alpha -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Recombinant fragment corresponding to Human TNF alpha aa 77-233. E.coli derived Recombinant Human TNF-alpha
Database link: P01375 -
Positive control
- WB: Recombinant human TNF alpha protein (ab9642), SW480 cell lysate.
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General notes
This product is no longer batch tested in IHC, for an IHC validated antibody please see ab1793
Properties
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Form
Lyophilized:Reconstitute with 200µl of sterile water. -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
Storage buffer
No preservative, sterile filtered -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Light chain type
unknown -
Research areas
Images
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Sandwich ELISA - Anti-TNF alpha antibody (ab9635)
ab9635 detecting TNF alpha with sandwich ELISA
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Western blot - Anti-TNF alpha antibody (ab9635)Anti-TNF alpha antibody (ab9635) at 0.2 µg/ml + SW480 (Human colon adenocarcinoma cell line) Whole Cell Lysate at 10 µg
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Observed band size: 26 kDa why is the actual band size different from the predicted?
Additional bands at: 48 kDa. We are unsure as to the identity of these extra bands.
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Western blot - Anti-TNF alpha antibody (ab9635)
