Anti-TMUB2 antibody [EPR13741] - BSA and Azide free (ab250408)
![Anti-TMUB2 antibody [EPR13741] - BSA and Azide free (ab250408)](https://www.abcam.com/ps/products/250/ab250408/Images/ab250408-405848-anti-tmub2-antibody-epr13741-bsa-and-azide-free-western-blot.jpg "Anti-TMUB2 antibody [EPR13741] - BSA and Azide free (ab250408)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR13741] to TMUB2 - BSA and Azide free
- Suitable for: WB, IP, Flow Cyt
- Reacts with: Human
Overview
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Product name
Anti-TMUB2 antibody [EPR13741] - BSA and Azide free
See all TMUB2 primary antibodies -
Description
Rabbit monoclonal [EPR13741] to TMUB2 - BSA and Azide free -
Host species
Rabbit -
Specificity
Based on the sequence analysis, this antibody recognizes 4 isoforms with predicted MW of 34KDa, 32KDa, 19KDa and 10KDa, respectively.
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Tested applications
Suitable for: WB, IP, Flow Cytmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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General notes
Ab250408 is the carrier-free version of ab181183. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab250408 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Affinity purified -
Clonality
Monoclonal -
Clone number
EPR13741 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-TMUB2 antibody [EPR13741] - BSA and Azide free (ab250408)All lanes : Anti-TMUB2 antibody [EPR13741] (ab181183) at 1/1000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : HEK293 cell lysate
Lane 3 : HepG2 cell lysate
Lane 4 : A431 cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 34 kDa
Observed band size: 10-34 kDa why is the actual band size different from the predicted?This data was developed using ab181183, the same antibody clone in a different buffer formulation.
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![Flow Cytometry - Anti-TMUB2 antibody [EPR13741] - BSA and Azide free (ab250408)](https://www.abcam.com/ps/products/250/ab250408/Images/ab250408-2-anti-tmub2-antibody-epr13741-bsa-and-azide-free-flow-cytometry.jpg)
Flow Cytometry - Anti-TMUB2 antibody [EPR13741] - BSA and Azide free (ab250408) -
![Immunoprecipitation - Anti-TMUB2 antibody [EPR13741] - BSA and Azide free (ab250408)](https://www.abcam.com/ps/products/250/ab250408/Images/ab250408-399618-anti-tmub2-antibody-epr13741-bsa-and-azide-free-immunoprecipitation.jpg)
Immunoprecipitation - Anti-TMUB2 antibody [EPR13741] - BSA and Azide free (ab250408)This data was developed using ab181183, the same antibody clone in a different buffer formulation.
Western blot analysis on immunoprecipitation pellet from HeLa cell lysate (lane 1) or negative control (lane 2), labeling TMUB2 immunoprecipitated using ab181183 at 1/50 dilution and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.
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![Anti-TMUB2 antibody [EPR13741] - BSA and Azide free (ab250408)](https://www.abcam.com/ps/products/250/ab250408/Images/ab250408-4-benefits-of-recombinant-antibodies.png)
Anti-TMUB2 antibody [EPR13741] - BSA and Azide free (ab250408)