Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-TMPRSS2 antibody [EPR24407-87] - BSA and Azide free (ab280569)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR24407-87] to TMPRSS2 - BSA and Azide free
  • Suitable for: ICC, Flow Cyt
  • Reacts with: Human

Overview

  • Product name

    Anti-TMPRSS2 antibody [EPR24407-87] - BSA and Azide free
    See all TMPRSS2 primary antibodies

  • Description

    Rabbit monoclonal [EPR24407-87] to TMPRSS2 - BSA and Azide free

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • ICC: LNCaP cells. Flow Cyt: LNCaP cells.

  • General notes

    ab280569 is the carrier-free version of ab280567. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab280569 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunocytochemistry - Anti-TMPRSS2 antibody [EPR24407-87] - BSA and Azide free (ab280569)
    Immunocytochemistry - Anti-TMPRSS2 antibody [EPR24407-87] - BSA and Azide free (ab280569)

    This data was developed using ab280567, the same antibody clone in a different buffer formulation.

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized LNCaP and HEK-293 cells labelling TMPRSS2 with ab280567 at 1/50 dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green). Confocal image showing cytoplasmic and membranous staining in LNCaP cell line.

    Negative control: HEK-293 (PMID:20631123).

    ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (Red). The nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.

  • Flow Cytometry - Anti-TMPRSS2 antibody [EPR24407-87] - BSA and Azide free (ab280569)
    Flow Cytometry - Anti-TMPRSS2 antibody [EPR24407-87] - BSA and Azide free (ab280569)

    This data was developed using ab280567, the same antibody clone in a different buffer formulation.

    Flow cytometric analysis of HEK-293T (Human embryonic kidney epithelial cell, Left)/ LNCaP (Human prostate carcinoma epithelial cell, Right) cells labelling TMPRSS2 with ab280567 at 1/500 dilution (0.1µg)/ red compared with Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat anti rabbit IgG (Alexa Fluor® 647, ab150079) at 1/2000 dilution was used as the secondary antibody.

    Negative control: HEK-239T (PMID: 20631123).

    Gated on viable cells.

  • Anti-TMPRSS2 antibody [EPR24407-87] - BSA and Azide free (ab280569)
    Anti-TMPRSS2 antibody [EPR24407-87] - BSA and Azide free (ab280569)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

© 2021 Astana Biomed Group LLP. All rights reserved.