Anti-TMPRSS2 antibody [EPR24407-87] - BSA and Azide free (ab280569)
![Anti-TMPRSS2 antibody [EPR24407-87] - BSA and Azide free (ab280569)](https://www.abcam.com/ps/products/280/ab280569/Images/ab280569-1-anti-tmprss2-antibody-epr2440787-immunocytochemistry-lncap.jpg "Anti-TMPRSS2 antibody [EPR24407-87] - BSA and Azide free (ab280569)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR24407-87] to TMPRSS2 - BSA and Azide free
- Suitable for: ICC, Flow Cyt
- Reacts with: Human
Overview
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Product name
Anti-TMPRSS2 antibody [EPR24407-87] - BSA and Azide free
See all TMPRSS2 primary antibodies -
Description
Rabbit monoclonal [EPR24407-87] to TMPRSS2 - BSA and Azide free -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt HumanICC Human
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Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- ICC: LNCaP cells. Flow Cyt: LNCaP cells.
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General notes
ab280569 is the carrier-free version of ab280567. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab280569 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
Constituent: 100% PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR24407-87 -
Isotype
IgG -
Research areas
Images
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Immunocytochemistry - Anti-TMPRSS2 antibody [EPR24407-87] - BSA and Azide free (ab280569)
This data was developed using ab280567, the same antibody clone in a different buffer formulation.
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized LNCaP and HEK-293 cells labelling TMPRSS2 with ab280567 at 1/50 dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green). Confocal image showing cytoplasmic and membranous staining in LNCaP cell line.
Negative control: HEK-293 (PMID:20631123).
ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (Red). The nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.
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![Flow Cytometry - Anti-TMPRSS2 antibody [EPR24407-87] - BSA and Azide free (ab280569)](https://www.abcam.com/ps/products/280/ab280569/Images/ab280569-2-anti-tmprss2-antibody-epr2440787-flow-cytometry-293t.jpg)
Flow Cytometry - Anti-TMPRSS2 antibody [EPR24407-87] - BSA and Azide free (ab280569)This data was developed using ab280567, the same antibody clone in a different buffer formulation.
Flow cytometric analysis of HEK-293T (Human embryonic kidney epithelial cell, Left)/ LNCaP (Human prostate carcinoma epithelial cell, Right) cells labelling TMPRSS2 with ab280567 at 1/500 dilution (0.1µg)/ red compared with Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat anti rabbit IgG (Alexa Fluor® 647, ab150079) at 1/2000 dilution was used as the secondary antibody.
Negative control: HEK-239T (PMID: 20631123).
Gated on viable cells.
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![Anti-TMPRSS2 antibody [EPR24407-87] - BSA and Azide free (ab280569)](https://www.abcam.com/ps/products/280/ab280569/Images/ab280569-3-Benefits-of-Recombinant-antibodies.jpg)
Anti-TMPRSS2 antibody [EPR24407-87] - BSA and Azide free (ab280569)
