Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Tmem27 antibody [EPR17748] - BSA and Azide free (ab240356)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR17748] to Tmem27 - BSA and Azide free
  • Suitable for: Flow Cyt (Intra), WB, ICC/IF, IHC-P
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-Tmem27 antibody [EPR17748] - BSA and Azide free
    See all Tmem27 primary antibodies

  • Description

    Rabbit monoclonal [EPR17748] to Tmem27 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: Flow Cyt (Intra), WB, ICC/IF, IHC-Pmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    ab240356 is the carrier-free version of ab200664.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunocytochemistry/ Immunofluorescence - Anti-Tmem27 antibody [EPR17748] - BSA and Azide free (ab240356)
    Immunocytochemistry/ Immunofluorescence - Anti-Tmem27 antibody [EPR17748] - BSA and Azide free (ab240356)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HEK293 (Human embryonic kidney) cells labeling Tmem27 with ab200664 at 1/500 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/500 dilution (green).

    Cytoplasm staining on HEK293 cell line is observed.

    The nuclear counter stain is DAPI (blue).

    Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution (red).

    The negative controls are as follows:
    -ve control 1: ab200664 at 1/500 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution.
    -ve control 2: ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/500 dilution.

     

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab200664).

  • Immunocytochemistry/ Immunofluorescence - Anti-Tmem27 antibody [EPR17748] - BSA and Azide free (ab240356)
    Immunocytochemistry/ Immunofluorescence - Anti-Tmem27 antibody [EPR17748] - BSA and Azide free (ab240356)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized BxPC-3 (Human pancreas adenocarcinoma cells) cells labeling Tmem27 with ab200664 at 1/500 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/500 dilution (green).

    Cytoplasm staining on BxPC-3 cell line is observed.

    The nuclear counter stain is DAPI (blue).

    Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution (red).
    The negative controls are as follows:
    -ve control 1: ab200664 at 1/500 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution.
    -ve control 2: ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/500 dilution.

     

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab200664).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tmem27 antibody [EPR17748] - BSA and Azide free (ab240356)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tmem27 antibody [EPR17748] - BSA and Azide free (ab240356)

    Immunohistochemical analysis of paraffin-embedded Rat kidney tissue labeling Tmem27 with ab200664 at 1/10000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution.

    Luminal surface and cytoplasm staining on rat kidney tissue is observed.

    Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab200664).

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tmem27 antibody [EPR17748] - BSA and Azide free (ab240356)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tmem27 antibody [EPR17748] - BSA and Azide free (ab240356)

    Immunohistochemical analysis of paraffin-embedded Human cerebral cortex tissue labeling Tmem27 with ab200664 at 1/10000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution.

    No staining on Human cerebral cortex tissue is observed (Negative control for Tmem27).

    Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab200664).

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Flow Cytometry - Anti-Tmem27 antibody [EPR17748] - BSA and Azide free (ab240356)
    Flow Cytometry - Anti-Tmem27 antibody [EPR17748] - BSA and Azide free (ab240356)

    Flow cytometric analysis of 2% paraformaldehyde-fixed BxPC-3 (Human pancreas adenocarcinoma cells) cells labeling Tmem27 with ab200664 at 1/100 dilution (red) compared with a rabbit monoclonal IgG isotype control (ab172730; black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody; blue). Goat anti rabbit IgG (FITC) at 1/150 dilution was used as the secondary antibody.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab200664).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tmem27 antibody [EPR17748] - BSA and Azide free (ab240356)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tmem27 antibody [EPR17748] - BSA and Azide free (ab240356)

    Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling Tmem27 with ab200664 at 1/10000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution.

    Luminal surface and cytoplasm staining on Human kidney tissue is observed.

    Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab200664).

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Anti-Tmem27 antibody [EPR17748] - BSA and Azide free (ab240356)
    Anti-Tmem27 antibody [EPR17748] - BSA and Azide free (ab240356)

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