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Anti-Thrombopoietin antibody [EPR14948] - BSA and Azide free (ab251218)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR14948] to Thrombopoietin - BSA and Azide free
  • Suitable for: Flow Cyt, WB, ICC, IHC-P
  • Reacts with: Human

Overview

  • Product name

    Anti-Thrombopoietin antibody [EPR14948] - BSA and Azide free
    See all Thrombopoietin primary antibodies

  • Description

    Rabbit monoclonal [EPR14948] to Thrombopoietin - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: Flow Cyt, WB, ICC, IHC-Pmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    Ab251218 is the carrier-free version of ab196026. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab251218 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thrombopoietin antibody [EPR14948] - BSA and Azide free (ab251218)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thrombopoietin antibody [EPR14948] - BSA and Azide free (ab251218)

    This data was developed using ab196026, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Human bone marrow tissue labeling Thrombopoietin with ab196026 at 1/4000 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution and counter-stained with Hematoxylin. (inset: negative control). Note: Cytoplasm staining on human bone marrow tissue was observed. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
  • Flow Cytometry - Anti-Thrombopoietin antibody [EPR14948] - BSA and Azide free (ab251218)
    Flow Cytometry - Anti-Thrombopoietin antibody [EPR14948] - BSA and Azide free (ab251218)
    This data was developed using ab196026, the same antibody clone in a different buffer formulation.Flow Cytometry analysis of Jurkat (human acute T cell leukemia) labelling Thrombopoietin with purified ab196026 at 1/40 (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Alexa Fluor® 488 goat anti-rabbit IgG (1/2000) was used as the secondary antibody. Black - Isotype control, rabbit monoclonal IgG. Blue - Unlabelled control, cells without incubation with primary and secondary antibodies.
  • Immunocytochemistry - Anti-Thrombopoietin antibody [EPR14948] - BSA and Azide free (ab251218)
    Immunocytochemistry - Anti-Thrombopoietin antibody [EPR14948] - BSA and Azide free (ab251218)
    This data was developed using ab196026, the same antibody clone in a different buffer formulation.Immunofluorescent analysis of Jurkat cells (4% Paraformaldehyde-fixed, 0.1% tritonX-100 permeabilized) labeling Thrombopoietin with ab196026 at 1/50 dilution followed by Goat anti rabbit IgG (AlexaFluor® 488) (ab150077) secondary at 1/400 dilution and counter-stained with DAPI (blue).Note: Cytoplasm staining on Jurkat cell line was observed.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thrombopoietin antibody [EPR14948] - BSA and Azide free (ab251218)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thrombopoietin antibody [EPR14948] - BSA and Azide free (ab251218)
    This data was developed using ab196026, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling Thrombopoietin with ab196026 at 1/4000 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution and counter-stained with Hematoxylin. (inset: negative control). Note: Cytoplasm staining on human liver tissue was observed. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
  • Western blot - Anti-Thrombopoietin antibody [EPR14948] - BSA and Azide free (ab251218)
    Western blot - Anti-Thrombopoietin antibody [EPR14948] - BSA and Azide free (ab251218)
    Anti-Thrombopoietin antibody [EPR14948] (ab196026) at 1/5000 dilution + Thrombopoietin recombinant protein at 0.01 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

    Predicted band size: 38 kDa



    This data was developed using ab196026, the same antibody clone in a different buffer formulation.

  • Western blot - Anti-Thrombopoietin antibody [EPR14948] - BSA and Azide free (ab251218)
    Western blot - Anti-Thrombopoietin antibody [EPR14948] - BSA and Azide free (ab251218)
    Anti-Thrombopoietin antibody [EPR14948] (ab196026) at 1/1000 dilution + Human fetal liver at 10 µg

    Secondary
    Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

    Predicted band size: 38 kDa



    This data was developed using ab196026, the same antibody clone in a different buffer formulation.

     

  • Anti-Thrombopoietin antibody [EPR14948] - BSA and Azide free (ab251218)
    Anti-Thrombopoietin antibody [EPR14948] - BSA and Azide free (ab251218)

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