Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Thrombomodulin antibody [EPR18217] (ab187075)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR18217] to Thrombomodulin
  • Suitable for: WB, IHC-P, Flow Cyt
  • Reacts with: Mouse, Rat

Overview

  • Product name

    Anti-Thrombomodulin antibody [EPR18217]
    See all Thrombomodulin primary antibodies

  • Description

    Rabbit monoclonal [EPR18217] to Thrombomodulin

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Mouse
    IHC-P
    Mouse
    Rat
    WB
    Mouse
    Rat
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: bEND.3 cell lysate; Rat lung and heart lysates; Mouse lung and placenta lysates. IHC-P: Mouse and rat lung tissues. Flow Cyt: bEND.3 cells.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-Thrombomodulin antibody [EPR18217] (ab187075)
    Western blot - Anti-Thrombomodulin antibody [EPR18217] (ab187075)
    All lanes : Anti-Thrombomodulin antibody [EPR18217] (ab187075) at 1/1000 dilution

    Lane 1 : Rat heart lysate
    Lane 2 : bEND.3 (mouse brain endothelioma cell line) whole cell lysate
    Lane 3 : Mouse placenta lysate
    Lane 4 : Rat lung lysate
    Lane 5 : Mouse lung lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Developed using the ECL technique.

    Predicted band size: 62 kDa
    Observed band size: 105,75 kDa
    why is the actual band size different from the predicted?



     

    Exposure time : Lane 1: 3 minutes; Lane 2: 15 seconds; Lane 3: 30 seconds; Lanes 4-5: 3 seconds.

    Blocking/Dilution buffer: 5% NFDM/TBST.

    The molecular weight observed is consistent with what has been described in the literature (PMID: 9619877).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thrombomodulin antibody [EPR18217] (ab187075)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thrombomodulin antibody [EPR18217] (ab187075)

    Immunohistochemical analysis of paraffin-embedded mouse lung tissue labeling Thrombomodulin with ab187075 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Positive staining on endothelial cells of mouse lung (PMID: 23946288). Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thrombomodulin antibody [EPR18217] (ab187075)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thrombomodulin antibody [EPR18217] (ab187075)

    Immunohistochemical analysis of paraffin-embedded rat lung tissue labeling Thrombomodulin with ab187075 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Positive staining on endothelial cells of rat lung (PMID: 23946288). Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Flow Cytometry - Anti-Thrombomodulin antibody [EPR18217] (ab187075)
    Flow Cytometry - Anti-Thrombomodulin antibody [EPR18217] (ab187075)

    Flow cytometric analysis of bEND.3 (mouse brain endothelioma cell line) cell line labeling Thrombomodulin with ab187075 at 1/60 dilution (red) compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) at 1/2000 dilution was used as the secondary antibody.

    Total viable cells were gated for the FC image.

  • Anti-Thrombomodulin antibody [EPR18217] (ab187075)
    Anti-Thrombomodulin antibody [EPR18217] (ab187075)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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