Antibodies, Proteins, Kits and Reagents for Life Science

291 484 ₸ Product size

100 µl 291 484 ₸

Order now and get it on Tuesday March 09, 2021

Anti-Thrombomodulin antibody [EPR18217] (ab187075)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR18217] to Thrombomodulin
Suitable for: WB, IHC-P, Flow Cyt
Reacts with: Mouse, Rat

Product name

Anti-Thrombomodulin antibody [EPR18217]
See all Thrombomodulin primary antibodies
Description

Rabbit monoclonal [EPR18217] to Thrombomodulin
Host species

Rabbit

Tested Applications & Species

Application	Species
Flow Cyt	Mouse
IHC-P	Mouse Rat
WB	Mouse Rat

See all applications and species data

Immunogen

Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: bEND.3 cell lysate; Rat lung and heart lysates; Mouse lung and placenta lysates. IHC-P: Mouse and rat lung tissues. Flow Cyt: bEND.3 cells.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 0.05% BSA, 40% Glycerol (glycerin, glycerine), PBS
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR18217
Isotype

IgG
Research areas

Western blot - Anti-Thrombomodulin antibody [EPR18217] (ab187075)

All lanes : Anti-Thrombomodulin antibody [EPR18217] (ab187075) at 1/1000 dilution

Lane 1 : Rat heart lysate
Lane 2 : bEND.3 (mouse brain endothelioma cell line) whole cell lysate
Lane 3 : Mouse placenta lysate
Lane 4 : Rat lung lysate
Lane 5 : Mouse lung lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Developed using the ECL technique.

Predicted band size: 62 kDa
Observed band size: 105,75 kDa
why is the actual band size different from the predicted?

Exposure time : Lane 1: 3 minutes; Lane 2: 15 seconds; Lane 3: 30 seconds; Lanes 4-5: 3 seconds.

Blocking/Dilution buffer: 5% NFDM/TBST.

The molecular weight observed is consistent with what has been described in the literature (PMID: 9619877).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thrombomodulin antibody [EPR18217] (ab187075)

Immunohistochemical analysis of paraffin-embedded mouse lung tissue labeling Thrombomodulin with ab187075 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Positive staining on endothelial cells of mouse lung (PMID: 23946288). Counter stained with Hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thrombomodulin antibody [EPR18217] (ab187075)

Immunohistochemical analysis of paraffin-embedded rat lung tissue labeling Thrombomodulin with ab187075 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Positive staining on endothelial cells of rat lung (PMID: 23946288). Counter stained with Hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Flow Cytometry - Anti-Thrombomodulin antibody [EPR18217] (ab187075)

Flow cytometric analysis of bEND.3 (mouse brain endothelioma cell line) cell line labeling Thrombomodulin with ab187075 at 1/60 dilution (red) compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) at 1/2000 dilution was used as the secondary antibody.

Total viable cells were gated for the FC image.
Anti-Thrombomodulin antibody [EPR18217] (ab187075)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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