Antibodies, Proteins, Kits and Reagents for Life Science

284 784 ₸ Product size

100 µl 284 784 ₸

Order now and get it on Tuesday March 09, 2021

Anti-Thrombomodulin antibody [EPR18217-209] (ab230010)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR18217-209] to Thrombomodulin
Suitable for: IHC-Fr, ICC/IF, IP, Flow Cyt, IHC-P, WB
Reacts with: Mouse

Product name

Anti-Thrombomodulin antibody [EPR18217-209]
See all Thrombomodulin primary antibodies
Description

Rabbit monoclonal [EPR18217-209] to Thrombomodulin
Host species

Rabbit

Tested Applications & Species

Application	Species
Flow Cyt	Mouse
ICC/IF	Mouse
IHC-Fr	Mouse
IHC-P	Mouse
IP	Mouse
WB	Mouse

See all applications and species data

Immunogen

Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: Mouse lung and placenta tissue lysates; bEND.3 whole cell lysate. IHC-P: Mouse lung and stomach tissues. IHC-Fr: Mouse embryo E14.5 (developing lung) tissue. ICC/IF: bEND.3 cells. Flow Cyt: bEND.3 cells. IP: bEND.3 whole cell lysate; Mouse lung tissue lysate.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR18217-209
Isotype

IgG
Research areas

Western blot - Anti-Thrombomodulin antibody [EPR18217-209] (ab230010)

All lanes : Anti-Thrombomodulin antibody [EPR18217-209] (ab230010) at 1/1000 dilution

Lane 1 : Mouse lung tissue lysate at 20 µg
Lane 2 : bEND.3 (mouse brain endothelioma cell line) whole cell lysate at 10 µg
Lane 3 : Mouse placenta tissue lysate at 10 µg

Secondary
Lane 1 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Lanes 2-3 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution

Developed using the ECL technique.

Predicted band size: 62 kDa
Observed band size: 105,75 kDa
why is the actual band size different from the predicted?

Exposure times: Lane 1: 1 minute; Lane 2: 20 seconds; Lane 3: 10 seconds.

Blocking/Dilution buffer: 5% NFDM/TBST.

The blot was developed on a BIO-RAD® ChemiDoc™ MP instrument.
Immunocytochemistry/ Immunofluorescence - Anti-Thrombomodulin antibody [EPR18217-209] (ab230010)

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized bEND.3 (mouse brain endothelioma cell line) cells labeling Thrombomodulin with ab230010 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic and membranous staining in bEND.3 cell line (PMID: 7622601; PMID: 8223719).

The nuclear counterstain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594) (ab195889) at 1/200 dilution (red).

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution.
Immunoprecipitation - Anti-Thrombomodulin antibody [EPR18217-209] (ab230010)

Thrombomodulin was immunoprecipitated from 0.35 mg of mouse lung tissue lysate with ab230010 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab230010 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/5000 dilution.

Lane 1: Mouse lung tissue lysate 10 μg (Input).
Lane 2: ab230010 IP in mouse lung tissue lysate.
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab230010 in mouse lung tissue lysate.

Exposure time: 10 seconds.

Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Immunoprecipitation - Anti-Thrombomodulin antibody [EPR18217-209] (ab230010)

Thrombomodulin was immunoprecipitated from 0.35 mg of bEND.3 (mouse brain endothelioma cell line) whole cell lysate with ab230010 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab230010 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/5000 dilution.

Lane 1: bEND.3 whole cell lysate 10 μg (Input).
Lane 2: ab230010 IP in bEND.3 whole cell lysate.
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab230010 in bEND.3 whole cell lysate.

Exposure time: 10 seconds.

Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Flow Cytometry - Anti-Thrombomodulin antibody [EPR18217-209] (ab230010)

Flow cytometric analysis of bEND.3 (mouse brain endothelioma cell line) cells labeling Thrombomodulin with ab230010 at 1/500 dilution (red) compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077), at 1/2000 dilution was used as the secondary antibody.

Gated on total viable cells.
Immunohistochemistry (Frozen sections) - Anti-Thrombomodulin antibody [EPR18217-209] (ab230010)

Immunohistochemical analysis of 4% paraformaldehyde-fixed, 0.2% Triton X-100 permeabilized frozen mouse embryo E14.5 (developing lung) tissue labeling Thrombomodulin with ab230010 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) at 1/1000 dilution (green). Positive membrane staining in the developing lung in mouse E14.5 embryo (PMID: 28306049) is observed.

The nuclear counterstain is DAPI (blue).

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) at 1/1000 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thrombomodulin antibody [EPR18217-209] (ab230010)

Immunohistochemical analysis of paraffin-embedded mouse lung tissue labeling Thrombomodulin with ab230010 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Positive staining on endothelial cells of mouse lung (PMID: 23946288; PMID: 10231031) is observed. Counter stained with Hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thrombomodulin antibody [EPR18217-209] (ab230010)

Immunohistochemical analysis of paraffin-embedded mouse stomach tissue labeling Thrombomodulin with ab230010 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Positive staining on endothelial cells of mouse stomach (PMID: 23946288; PMID: 10231031) is observed. Counter stained with Hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Anti-Thrombomodulin antibody [EPR18217-209] (ab230010)

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