Anti-THOC2/Tho2 antibody (ab46685)
Key features and details
- Rabbit polyclonal to THOC2/Tho2
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-THOC2/Tho2 antibody
See all THOC2/Tho2 primary antibodies -
Description
Rabbit polyclonal to THOC2/Tho2 -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Zebrafish
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Immunogen
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Positive control
- This antibody gave a positive signal in Hela Nuclear cell lysate.
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General notes
This product was previously labelled as THOC2
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab46685 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application Abreviews Notes WB Use a concentration of 1 µg/ml. Detects a band of approximately 183 kDa (predicted molecular weight: 183 kDa). ICC/IF Use a concentration of 1 µg/ml. Target
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Function
Component of the THO subcomplex of the TREX complex. The TREX complex specifically associates with spliced mRNA and not with unspliced pre-mRNA. It is recruited to spliced mRNAs by a transcription-independent mechanism. Binds to mRNA upstream of the exon-junction complex (EJC) and is recruited in a splicing- and cap-dependent manner to a region near the 5' end of the mRNA where it functions in mRNA export. The recruitment occurs via an interaction between THOC4 and the cap-binding protein NCBP1. UAP56 functions as a bridge between THOC4 and the THO complex.The TREX complex is essential for the export of Kaposi's sarcoma-associated herpesvirus (KSHV) intronless mRNAs and infectious virus production. The recruitment of the TREX complex to the intronless viral mRNA occurs via an interaction between KSHV ORF57 protein and THOC4. -
Sequence similarities
Belongs to the THOC2 family. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 57187 Human
- Entrez Gene: 331401 Mouse
- Entrez Gene: 368417 Zebrafish
- Omim: 300395 Human
- SwissProt: Q8NI27 Human
- SwissProt: B1AZI6 Mouse
- SwissProt: Q6DRE1 Zebrafish
- Unigene: 149991 Human
see all -
Alternative names
- CXorf3 antibody
- DJ506G2.1 antibody
- hTREX120 antibody
see all
Images
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Western blot - Anti-THOC2/Tho2 antibody (ab46685)Anti-THOC2/Tho2 antibody (ab46685) at 1 µg/ml + HeLa (Human epithelial carcinoma cell line) Nuclear Lysate at 10 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Performed under reducing conditions.
Predicted band size: 183 kDa
Observed band size: 183 kDa
Additional bands at: 130 kDa, 35 kDa, 55 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 1 minute -
Immunocytochemistry/ Immunofluorescence - Anti-THOC2/Tho2 antibody (ab46685)ICC/IF image of ab46685 stained HeLa cells. The cells were 4% PFA fixed (10 min), permabilised in 0.1% PBS-Tween (20 min) and incubated with the antibody (ab46685, 1µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue). This antibody also gave a positive IF result in HepG2, Hek293 and MCF7 cells fixed in 4% PFA and HepG2 cells fixed in 100% methanol at 1ug/ml. However, this Fast-Track antibody is not yet fully characterised. This image represents inconclusive preliminary data.
Datasheets and documents
References (0)
ab46685 has not yet been referenced specifically in any publications.
Images
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Western blot - Anti-THOC2/Tho2 antibody (ab46685)Anti-THOC2/Tho2 antibody (ab46685) at 1 µg/ml + HeLa (Human epithelial carcinoma cell line) Nuclear Lysate at 10 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Performed under reducing conditions.
Predicted band size: 183 kDa
Observed band size: 183 kDa
Additional bands at: 130 kDa, 35 kDa, 55 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 1 minute
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Immunocytochemistry/ Immunofluorescence - Anti-THOC2/Tho2 antibody (ab46685)ICC/IF image of ab46685 stained HeLa cells. The cells were 4% PFA fixed (10 min), permabilised in 0.1% PBS-Tween (20 min) and incubated with the antibody (ab46685, 1µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue). This antibody also gave a positive IF result in HepG2, Hek293 and MCF7 cells fixed in 4% PFA and HepG2 cells fixed in 100% methanol at 1ug/ml. However, this Fast-Track antibody is not yet fully characterised. This image represents inconclusive preliminary data.
