Antibodies, Proteins, Kits and Reagents for Life Science

307 566 ₸ Product size

100 µl 307 566 ₸

Order now and get it on Friday March 19, 2021

Anti-Tetraspanin-33 antibody [EPR23121-146] (ab238140)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR23121-146] to Tetraspanin-33
Suitable for: Flow Cyt, Dot blot
Reacts with: Human, Recombinant fragment

Product name

Anti-Tetraspanin-33 antibody [EPR23121-146]
See all Tetraspanin-33 primary antibodies
Description

Rabbit monoclonal [EPR23121-146] to Tetraspanin-33
Host species

Rabbit
Tested Applications & Species

Application Species
Dot
Recombinant fragment

Flow Cyt
Human

See all applications and species data
Immunogen

Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
Positive control
- Flow cyt: Human PBMCs.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

Learn more here.

Application	Species
Dot	Recombinant fragment
Flow Cyt	Human

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR23121-146
Isotype

IgG
Research areas

Flow Cytometry - Anti-Tetraspanin-33 antibody [EPR23121-146] (ab238140)

Flow cytometric analysis of human peripheral blood mononuclear cells (PBMC) treated with 1ug/ml human CD40 ligand and 100U/ml human IL-4 for 72h labeling Tetraspanin-33 with ab238140 at 1/500 dilution (Right) compared with a Rabbit monoclonal IgG Isotype control (ab172730) (Left). Goat anti-rabbit IgG (Alexa Fluor^® 488, ab150077), at 1/2000 dilution was used as the secondary antibody.

Cells were stained with rabbit IgG (Left) or ab238140 (Right). Then stained with anti-CD19 conjugated to Alexa Fluor^® 647.

Gated on viable cells.
Dot Blot - Anti-Tetraspanin-33 antibody [EPR23121-146] (ab238140)

Dot blot analysis of Tetraspanin-33 labeled with ab238140 at 1/1000 dilution.

Lane 1: Recombinant tetraspanin-33 fragment containing the sequence at the immunogen region.
Lane 2: BSA.

Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution was used as secondary antibody.

Blocking and dilution buffer: 5% NFDM/TBST.

Exposure time: 3 minutes.
Anti-Tetraspanin-33 antibody [EPR23121-146] (ab238140)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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