Anti-Tetraspanin-33 antibody [EPR23121-146] (ab238140)
![Anti-Tetraspanin-33 antibody [EPR23121-146] (ab238140)](https://www.abcam.com/ps/products/238/ab238140/Images/ab238140-389959-anti-tetraspanin33-antibody-epr23121146-flow-cytometry-human-peripheral-blood-mononuclear-cell.jpg "Anti-Tetraspanin-33 antibody [EPR23121-146] (ab238140)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR23121-146] to Tetraspanin-33
- Suitable for: Flow Cyt, Dot blot
- Reacts with: Human, Recombinant fragment
Overview
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Product name
Anti-Tetraspanin-33 antibody [EPR23121-146]
See all Tetraspanin-33 primary antibodies -
Description
Rabbit monoclonal [EPR23121-146] to Tetraspanin-33 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species Dot Recombinant fragmentFlow Cyt Human
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Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- Flow cyt: Human PBMCs.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR23121-146 -
Isotype
IgG -
Research areas
Images
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Flow Cytometry - Anti-Tetraspanin-33 antibody [EPR23121-146] (ab238140)
Flow cytometric analysis of human peripheral blood mononuclear cells (PBMC) treated with 1ug/ml human CD40 ligand and 100U/ml human IL-4 for 72h labeling Tetraspanin-33 with ab238140 at 1/500 dilution (Right) compared with a Rabbit monoclonal IgG Isotype control (ab172730) (Left). Goat anti-rabbit IgG (Alexa Fluor® 488, ab150077), at 1/2000 dilution was used as the secondary antibody.
Cells were stained with rabbit IgG (Left) or ab238140 (Right). Then stained with anti-CD19 conjugated to Alexa Fluor® 647.
Gated on viable cells.
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![Dot Blot - Anti-Tetraspanin-33 antibody [EPR23121-146] (ab238140)](https://www.abcam.com/ps/products/238/ab238140/Images/ab238140-389958-anti-tetraspanin33-antibody-epr23121146-dot-blot.jpg)
Dot Blot - Anti-Tetraspanin-33 antibody [EPR23121-146] (ab238140)Dot blot analysis of Tetraspanin-33 labeled with ab238140 at 1/1000 dilution.
Lane 1: Recombinant tetraspanin-33 fragment containing the sequence at the immunogen region.
Lane 2: BSA.Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution was used as secondary antibody.
Blocking and dilution buffer: 5% NFDM/TBST.
Exposure time: 3 minutes.
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![Anti-Tetraspanin-33 antibody [EPR23121-146] (ab238140)](https://www.abcam.com/ps/products/238/ab238140/Images/ab238140-3-benefits-of-recombinant-antibodies.png)
Anti-Tetraspanin-33 antibody [EPR23121-146] (ab238140)