Anti-Tetraspanin-33 antibody (ab79130)
Key features and details
- Rabbit polyclonal to Tetraspanin-33
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Tetraspanin-33 antibody
See all Tetraspanin-33 primary antibodies -
Description
Rabbit polyclonal to Tetraspanin-33 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Cow, Zebrafish
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Immunogen
Synthetic peptide corresponding to Human Tetraspanin-33 aa 250 to the C-terminus (C terminal) conjugated to keyhole limpet haemocyanin.
(Peptide available asab87705) -
Positive control
- This antibody gave a positive signal in Human Spleen Tissue Lysate.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Immunizing Peptide (Blocking)
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab79130 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application Abreviews Notes WB Use a concentration of 1 mg/ml. Detects a band of approximately 32 kDa (predicted molecular weight: 32 kDa). IHC-P Use a concentration of 5 µg/ml. Target
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Function
Plays an important role in normal erythropoiesis. It has a role in the differentiation of erythroid progenitors. -
Tissue specificity
Predominantly expressed in erythroblasts. -
Sequence similarities
Belongs to the tetraspanin (TM4SF) family. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 340348 Human
- Entrez Gene: 232670 Mouse
- Entrez Gene: 436890 Zebrafish
- Omim: 610120 Human
- SwissProt: Q3SYV5 Cow
- SwissProt: Q86UF1 Human
- SwissProt: Q8R3S2 Mouse
- SwissProt: Q5RH71 Zebrafish
see all -
Alternative names
- 1300010A20Rik antibody
- AI035228 antibody
- hPen antibody
see all
Images
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Western blot - Anti-Tetraspanin-33 antibody (ab79130)Anti-Tetraspanin-33 antibody (ab79130) at 1 µg/ml + Human spleen tissue lysate - total protein (ab29699) at 10 µg
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 32 kDa
Observed band size: 30 kDa why is the actual band size different from the predicted?
Exposure time: 5 minutes -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tetraspanin-33 antibody (ab79130)IHC image of ab79130 staining in Hodgkin's Lymphoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab79130, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
Protocols
Datasheets and documents
References (1)
ab79130 has been referenced in 1 publication.
- Luu VP et al. TSPAN33 is a novel marker of activated and malignant B cells. Clin Immunol 149:388-99 (2013). Human . PubMed: 24211713
Images
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Western blot - Anti-Tetraspanin-33 antibody (ab79130)Anti-Tetraspanin-33 antibody (ab79130) at 1 µg/ml + Human spleen tissue lysate - total protein (ab29699) at 10 µg
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 32 kDa
Observed band size: 30 kDa why is the actual band size different from the predicted?
Exposure time: 5 minutes
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tetraspanin-33 antibody (ab79130)
IHC image of ab79130 staining in Hodgkin's Lymphoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab79130, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.