Anti-TET3 antibody (ab153724)
Key features and details
- Rabbit polyclonal to TET3
- Suitable for: ICC/IF, IP, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-TET3 antibody
See all TET3 primary antibodies -
Description
Rabbit polyclonal to TET3 -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, IP, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Recombinant fragment, corresponding to a region within amino acids 1456-1660 of Human TET3 (Uniprot ID: O43151).
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunohistochemical analysis of paraffin-embedded mouse colon tissue staining TET3 protein at nucleus with ab153724 at 1/500 dilution.
Antigen Retrieval: EDTA based buffer, 15min.
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Immunofluorescence analysis of 4% paraformaldehyde-fixed (RT for 15 min) HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate labeling TET3 protein at cytoplasm and nucleus (green) with ab153724 at 1/400 dilution. Red: phalloidin, a cytoskeleton marker, at 1/50 dilution.
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Immunoprecipitation of TET3-transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cells.
IP Sample:
Lane 1: Transfected HEK-293T cell lysate.
Lane 2: Control with 2 µg of preimmune rabbit IgG
Lane 3: Immunoprecipitate of TET3-transfected HEK-293T cells using ab153724 at 2 µg.5% SDS-PAGE
Detection using ab153724 at 1/1000 dilution, followed by anti-rabbit IgG antibody.