Anti-Tet2 antibody - N-terminal (ab230358)
Key features and details
- Rabbit polyclonal to Tet2 - N-terminal
- Suitable for: IHC-P, WB, IP, ICC/IF, ChIP
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Tet2 antibody - N-terminal
See all Tet2 primary antibodies -
Description
Rabbit polyclonal to Tet2 - N-terminal -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WB, IP, ICC/IF, ChIPmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment within Human Tet2 (N terminal). The exact sequence is proprietary.
Database link: Q6N021 -
Positive control
- ChIP: U-2 OS chromatin extract; WB: HEK-293T, A431, HeLa and HepG2 whole cell lysate; DDDDK-human Tet2-transfected HEK-293T whole cell lysate; IHC-P:Human breast cancer and cervical carcinoma tissues; IF: HeLa cells; IP: HEK-293T whole cell lysate.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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ChIP - Anti-Tet2 antibody - N-terminal (ab230358)
Cross-linked ChIP was performed with U-2 OS (human bone osteosarcoma epithelial cell line) chromatin extract and 5 μg of either control rabbit IgG or ab230358. The precipitated DNA was detected by PCR with primer set targeting to CCND2.
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Western blot - Anti-Tet2 antibody - N-terminal (ab230358)All lanes : Anti-Tet2 antibody - N-terminal (ab230358) at 1/500 dilution
Lane 1 : HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 2 : A431 (Human epidermoid carcinoma cell line) whole cell lysate
Lane 3 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 4 : HepG2 (Human liver hepatocellular carcinoma cell line) whole cell lysate
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG
Predicted band size: 224 kDa5% SDS-PAGE gel.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tet2 antibody - N-terminal (ab230358)Paraffin-embedded human cervical carcinoma tissue stained for Tet2 using ab230358 at 1/500 dilution in immunohistochemical analysis.
Antigen Retrieval: EDTA based buffer, pH 8.0, 15min.
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Immunocytochemistry/ Immunofluorescence - Anti-Tet2 antibody - N-terminal (ab230358)Immunofluorescence analysis of 4% paraformaldehyde-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells labeling Tet2 (green) with ab230358 at 1/1000 dilution.
Counterstain: Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody at 1/1000 dilution. Blue: Hoechst 33342 staining.
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Western blot - Anti-Tet2 antibody - N-terminal (ab230358)All lanes : Anti-Tet2 antibody - N-terminal (ab230358) at 1/5000 dilution
Lane 1 : HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract
Lane 2 : DDDDK-human Tet2-transfected HEK-293T whole cell lysate
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG
Predicted band size: 224 kDa5% SDS-PAGE gel.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tet2 antibody - N-terminal (ab230358)Paraffin-embedded human breast cancer tissue stained for Tet2 using ab230358 at 1/500 dilution in immunohistochemical analysis.
Antigen Retrieval: EDTA based buffer, pH 8.0, 15min.
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Immunoprecipitation - Anti-Tet2 antibody - N-terminal (ab230358)Tet2 was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate with 5 µg ab230358. Western blot was performed from the immunoprecipitate using ab230358. Anti rabbit IgG antibody was used as secondary antibody.
Lane 1: Control IgG IP in HEK-293T whole cell lysate.
Lane 2: ab230358 IP in HEK-293T whole cell lysate.
