Anti-Tet2 antibody [EPR20546-135] - BSA and Azide free (ab277637)
![Anti-Tet2 antibody [EPR20546-135] - BSA and Azide free (ab277637)](https://www.abcam.com/ps/products/277/ab277637/Images/ab277637-450751-AntiTet2-antibody-epr20546135-western-blot-2.jpg "Anti-Tet2 antibody [EPR20546-135] - BSA and Azide free (ab277637)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR20546-135] to Tet2 - BSA and Azide free
- Suitable for: WB
- Knockout validated
- Reacts with: Mouse
Overview
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Product name
Anti-Tet2 antibody [EPR20546-135] - BSA and Azide free
See all Tet2 primary antibodies -
Description
Rabbit monoclonal [EPR20546-135] to Tet2 - BSA and Azide free -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Mouse
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: RAW264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage), whole cell lysate; Wild-type B-cell lysate.
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General notes
ab277637 is the carrier-free version of ab213369. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab277637 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
Constituent: 100% PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR20546-135 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Tet2 antibody [EPR20546-135] - BSA and Azide free (ab277637)All lanes : Anti-Tet2 antibody [EPR20546-135] (ab213369) at 1/1000 dilution
Lane 1 : Wild-type B-cell lysate
Lane 2 : Tet2/3 DKO B-cell lysate
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)
Predicted band size: 223 kDa
Observed band size: 257 kDa why is the actual band size different from the predicted?This data was developed using ab213369, the same antibody clone in a different buffer formulation.
Blocking and Diluting buffer: 5% NFDM/TBST
Exposure time: 60 seconds
This western was done using lysates from a Tet2/3 DKO B-cell model developed by Dr Vipul Shukla and Dr Chan-Wang Lio, in Prof Anjana Rao’s lab.
Image courtesy of Dr Hugo Sepulveda in Prof Anjana Rao’s laboratory (La Jolla Institute for Immunology). -
![Western blot - Anti-Tet2 antibody [EPR20546-135] - BSA and Azide free (ab277637)](https://www.abcam.com/ps/products/277/ab277637/Images/ab277637-1-anti-tet2-antibody-epr20546135-western-blot-murine.jpg)
Western blot - Anti-Tet2 antibody [EPR20546-135] - BSA and Azide free (ab277637)Anti-Tet2 antibody [EPR20546-135] (ab213369) at 1/1000 dilution + RAW264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage), whole cell lysate at 20 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)
Predicted band size: 223 kDa
Observed band size: 257 kDa why is the actual band size different from the predicted?This data was developed using ab213369, the same antibody clone in a different buffer formulation.
Blocking and Diluting buffer: 5% NFDM/TBST
Exposure time: 3 minutes
This blot was developed using a higher sensitivity ECL substrate.
