Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Tet2 antibody [EPR20546-135] - BSA and Azide free (ab277637)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR20546-135] to Tet2 - BSA and Azide free
  • Suitable for: WB
  • Knockout validated
  • Reacts with: Mouse

Overview

  • Product name

    Anti-Tet2 antibody [EPR20546-135] - BSA and Azide free
    See all Tet2 primary antibodies

  • Description

    Rabbit monoclonal [EPR20546-135] to Tet2 - BSA and Azide free

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    WB
    Mouse
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: RAW264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage), whole cell lysate; Wild-type B-cell lysate.

  • General notes

    ab277637 is the carrier-free version of ab213369. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab277637 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-Tet2 antibody [EPR20546-135] - BSA and Azide free (ab277637)
    Western blot - Anti-Tet2 antibody [EPR20546-135] - BSA and Azide free (ab277637)
    All lanes : Anti-Tet2 antibody [EPR20546-135] (ab213369) at 1/1000 dilution

    Lane 1 : Wild-type B-cell lysate
    Lane 2 : Tet2/3 DKO B-cell lysate

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

    Predicted band size: 223 kDa
    Observed band size: 257 kDa
    why is the actual band size different from the predicted?



    This data was developed using ab213369, the same antibody clone in a different buffer formulation.

    Blocking and Diluting buffer: 5% NFDM/TBST

    Exposure time: 60 seconds

    This western was done using lysates from a Tet2/3 DKO B-cell model developed by Dr Vipul Shukla and Dr Chan-Wang Lio, in Prof Anjana Rao’s lab.
    Image courtesy of Dr Hugo Sepulveda in Prof Anjana Rao’s laboratory (La Jolla Institute for Immunology).

  • Western blot - Anti-Tet2 antibody [EPR20546-135] - BSA and Azide free (ab277637)
    Western blot - Anti-Tet2 antibody [EPR20546-135] - BSA and Azide free (ab277637)
    Anti-Tet2 antibody [EPR20546-135] (ab213369) at 1/1000 dilution + RAW264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage), whole cell lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

    Predicted band size: 223 kDa
    Observed band size: 257 kDa why is the actual band size different from the predicted?



    This data was developed using ab213369, the same antibody clone in a different buffer formulation.

    Blocking and Diluting buffer: 5% NFDM/TBST

    Exposure time: 3 minutes

    This blot was developed using a higher sensitivity ECL substrate.

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