Anti-TCP1 eta antibody (ab240566)
Key features and details
- Rabbit polyclonal to TCP1 eta
- Suitable for: WB, IP
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-TCP1 eta antibody
See all TCP1 eta primary antibodies -
Description
Rabbit polyclonal to TCP1 eta -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IP HumanWB Human
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Immunogen
Synthetic peptide within Human TCP1 eta aa 493-543. The exact sequence is proprietary.
Database link: Q99832 -
Positive control
- WB: HeLa, HEK-293T, Jurkat, TCMK-1 and NIH/3T3 whole cell lysate. IP: HEK-293T whole cell lysate.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7-8 -
Concentration information loading... -
Purity
Affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-TCP1 eta antibody (ab240566)All lanes : Anti-TCP1 eta antibody (ab240566) at 0.1 µg/ml
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 3 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
Lane 4 : TCMK-1 (Mouse kidney epithelial cell line) whole cell lysate
Lane 5 : NIH/3T3 (Mouse embryo fibroblast cell line) whole cell lysate
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Predicted band size: 59 kDa
Exposure time: 3 seconds
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Immunoprecipitation - Anti-TCP1 eta antibody (ab240566)TCP1 eta was immunoprecipitated from of HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) using ab240566 at 6 μg per reaction. Western blot was performed on the immunoprecipitate using ab240566 at 0.4 μg/mL.
Lane 1: ab240566 IP in HEK-293T whole cell lysate.
Lane 2: Control IgG IP in HEK-293T whole cell lysate.Detection: Chemiluminescence with an exposure time of 3 seconds.
