Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Tbx6 antibody (ab38883)

Key features and details

  • Rabbit polyclonal to Tbx6
  • Suitable for: WB
  • Reacts with: Mouse, Human
  • Isotype: IgG

Overview

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab38883 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
WB
Mouse
Application Abreviews Notes
WB (1)
Use a concentration of 1 µg/ml. Detects a band of approximately 70 kDa (predicted molecular weight: 59 kDa).
Notes
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 70 kDa (predicted molecular weight: 59 kDa).

Target

  • Function

    Probable transcriptional regulator involved in developmental processes. Could be required for specification of paraxial mesoderm structures during gastrulation.

  • Tissue specificity

    Expressed in fetal tail bud, posterior spinal tissue, intervertebral disk and testis. Also expressed in adult testis, kidney, lung, muscle and thymus.

  • Sequence similarities

    Contains 1 T-box DNA-binding domain.

  • Developmental stage

    Expressed during gastrulation and during a second phase in some adult tissues.

  • Cellular localization

    Nucleus.
  • Information by UniProt

  • Database links

    • Alternative names

      • DFNB 67 antibody
      • DFNB67 antibody
      • SCDO5 antibody
      • T box 6 antibody
      • T box protein 6 antibody
      • T box transcription factor TBX 6 antibody
      • T box transcription factor TBX6 antibody
      • T-box protein 6 antibody
      • T-box transcription factor TBX6 antibody
      • Tbox 6 antibody
      • TBX 6 antibody
      • tbx6 antibody
      • TBX6_HUMAN antibody
      see all

    Images

    • Western blot - Anti-Tbx6 antibody (ab38883)
      Western blot - Anti-Tbx6 antibody (ab38883)
      All lanes : Anti-Tbx6 antibody (ab38883) at 1 µg/ml

      Lane 1 : Testis (Mouse) Tissue Lysate
      Lane 2 : Lung (Mouse) Tissue Lysate
      Lane 3 : Human testis tissue lysate - total protein (ab30257)
      Lane 4 : Lung (Human) Tissue Lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

      Performed under reducing conditions.

      Predicted band size: 59 kDa
      Observed band size: 50 kDa
      why is the actual band size different from the predicted?
      Additional bands at: 43 kDa. We are unsure as to the identity of these extra bands.


      Exposure time: 20 minutes

    References (4)

    Publishing research using ab38883? Please let us know so that we can cite the reference in this datasheet.

    ab38883 has been referenced in 4 publications.

    • Bredenkamp N  et al. Wnt Inhibition Facilitates RNA-Mediated Reprogramming of Human Somatic Cells to Naive Pluripotency. Stem Cell Reports 13:1083-1098 (2019). PubMed: 31708477
    • Liu C  et al. Suppressing Nodal Signaling Activity Predisposes Ectodermal Differentiation of Epiblast Stem Cells. Stem Cell Reports 11:43-57 (2018). PubMed: 30008328
    • Chal J  et al. Generation of human muscle fibers and satellite-like cells from human pluripotent stem cells in vitro. Nat Protoc 11:1833-50 (2016). PubMed: 27583644
    • Gouti M  et al. In vitro generation of neuromesodermal progenitors reveals distinct roles for wnt signalling in the specification of spinal cord and paraxial mesoderm identity. PLoS Biol 12:e1001937 (2014). ICC/IF ; Mouse . PubMed: 25157815

    Images

    • Western blot - Anti-Tbx6 antibody (ab38883)
      Western blot - Anti-Tbx6 antibody (ab38883)
      All lanes : Anti-Tbx6 antibody (ab38883) at 1 µg/ml

      Lane 1 : Testis (Mouse) Tissue Lysate
      Lane 2 : Lung (Mouse) Tissue Lysate
      Lane 3 : Human testis tissue lysate - total protein (ab30257)
      Lane 4 : Lung (Human) Tissue Lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

      Performed under reducing conditions.

      Predicted band size: 59 kDa
      Observed band size: 50 kDa
      why is the actual band size different from the predicted?
      Additional bands at: 43 kDa. We are unsure as to the identity of these extra bands.


      Exposure time: 20 minutes

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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