Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-TACI antibody [EPR23045-153] (ab239370)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR23045-153] to TACI
  • Suitable for: WB, IP, Flow Cyt
  • Reacts with: Human

Overview

  • Product name

    Anti-TACI antibody [EPR23045-153]
    See all TACI primary antibodies

  • Description

    Rabbit monoclonal [EPR23045-153] to TACI

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    IP
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: IM-9 and Raji whole cell lysates. Flow Cyt: Human peripheral blood mononuclear cells. IP: Raji whole cell lysate.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR23045-153

  • Isotype

    IgG

  • Research areas

Images

  • Western blot - Anti-TACI antibody [EPR23045-153] (ab239370)
    Western blot - Anti-TACI antibody [EPR23045-153] (ab239370)
    All lanes : Anti-TACI antibody [EPR23045-153] (ab239370) at 1/1000 dilution

    Lane 1 : IM-9 (human multiple myeloma B Lymphoblast), whole cell lysate
    Lane 2 : Raji (human Burkitt's lymphoma B lymphocyte), whole cell lysate
    Lane 3 : U937 (human histiocytic lymphoma monocyte), whole cell

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 32 kDa
    Observed band size: 32 kDa



    Blocking and dilution buffer: 5% NFDM/TBST.

    Exposure time: 136 seconds.

    Negative control: U937 (PMID: 11104810).

  • Flow Cytometry - Anti-TACI antibody [EPR23045-153] (ab239370)
    Flow Cytometry - Anti-TACI antibody [EPR23045-153] (ab239370)

    Flow cytometric analysis of human peripheral blood mononuclear cell (PBMC) cells labeling TACI with ab239370 at 1/400 dilution (Right) compared with a Rabbit monoclonal IgG (ab172730) isotype control (Left). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.

    Cells were stained with rabbit IgG (Left) or ab239370 (Right). Then stained with anti-CD19 conjugated to Alexa Fluor 647.

    Gated on viable cells.

  • Immunoprecipitation - Anti-TACI antibody [EPR23045-153] (ab239370)
    Immunoprecipitation - Anti-TACI antibody [EPR23045-153] (ab239370)

    TACI was immunoprecipitated from 0.35 mg Raji (Human Burkitt's lymphoma B lymphocyte) whole cell lysate 10µg with ab239370 at 1/30 dilution (2µg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab239370 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366) was used at 1/1000 dilution.

    Lane 1: Raji whole cell lysate 10µg

    Lane 2: ab239370 IP in Raji whole cell lysate

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab239370 in Raji whole cell lysate

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 30 seconds

     

  • Anti-TACI antibody [EPR23045-153] (ab239370)
    Anti-TACI antibody [EPR23045-153] (ab239370)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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