Anti-Syntaxin 7 antibody (ab192923)
Key features and details
- Rabbit polyclonal to Syntaxin 7
- Suitable for: IP, WB
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-Syntaxin 7 antibody
See all Syntaxin 7 primary antibodies -
Description
Rabbit polyclonal to Syntaxin 7 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IP HumanWB MouseHuman
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Immunogen
Synthetic peptide within Human Syntaxin 7 aa 100-200 (internal sequence). The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements. NP_003560.2.
Database link: O15400 -
Positive control
- HeLa, 293T, Jurkat, TCMK1 and NIH 3T3 whole cell lysates.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH 7-8 -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab192923 was affinity purified using an epitope specific to Syntaxin 7 immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunoprecipitation - Anti-Syntaxin 7 antibody (ab192923)
Detection of Syntaxin 7 by Western Blot of Immunprecipitate.
Lane 1: ab192923 at 1 µg/ml labeling Syntaxin 7 in 293T whole cell lysate immunoprecipitated using ab192923 at 6µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
Lane 2: Control IgG.
Detection: Chemiluminescence with exposure time of 30 seconds. -
Western blot - Anti-Syntaxin 7 antibody (ab192923)All lanes : Anti-Syntaxin 7 antibody (ab192923) at 0.1 µg/ml
Lane 1 : HeLa whole cell lysate
Lane 2 : 293T whole cell lysate
Lane 3 : Jurkat whole cell lysate
Lane 4 : TCMK1 whole cell lysate
Lane 5 : NIH 3T3 whole cell lysate
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Predicted band size: 29 kDa
Exposure time: 30 secondsLanes 1-3 prepared in RIPA lysis buffer; Lanes 4-5 prepared in NETN lysis buffer.
