Anti-Synphilin 1 antibody (ab6179)
Key features and details
- Rabbit polyclonal to Synphilin 1
- Suitable for: IHC-P
- Reacts with: Rat
- Isotype: IgG
Overview
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Product name
Anti-Synphilin 1 antibody
See all Synphilin 1 primary antibodies -
Description
Rabbit polyclonal to Synphilin 1 -
Host species
Rabbit -
Specificity
Immunohistochemical analysis of human brain indicates a high level of specificity for this antiserum. Cross reactivity: This antibody is known to react with rat and human. Cross reactivity with other species has not been tested yet. -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P Rat
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Immunogen
Synthetic peptide:
CASKGKNKAA
conjugated to KLH by a Glutaraldehyde linker, corresponding to amino acids 911-919 of Human Synphilin 1. -
General notes
This antibody stains Lewy bodies in Dorsal raphe nucleus of Parkinson’s diseased human brain.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Add glycerol to a final volume of 50% for extra stability and aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7 -
Concentration information loading... -
Purity
Whole antiserum -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Synphilin 1 antibody (ab6179)IHC image of ab6179 staining in rat normal brain formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab6179, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
