Anti-Syk (phospho Y526) + ZAP70 (phospho Y493) antibody [Zap70Y493-H11] (ab278675)
![Anti-Syk (phospho Y526) + ZAP70 (phospho Y493) antibody [Zap70Y493-H11] (ab278675)](https://www.abcam.com/ps/products/278/ab278675/Images/ab278675-4-zap70y493h113t3.jpg "Anti-Syk (phospho Y526) + ZAP70 (phospho Y493) antibody [Zap70Y493-H11] (ab278675)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [Zap70Y493-H11] to Syk (phospho Y526) + ZAP70 (phospho Y493)
- Suitable for: WB, Flow Cyt
- Reacts with: Mouse, Human
Overview
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Product name
Anti-Syk (phospho Y526) + ZAP70 (phospho Y493) antibody [Zap70Y493-H11] -
Description
Rabbit monoclonal [Zap70Y493-H11] to Syk (phospho Y526) + ZAP70 (phospho Y493) -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt HumanWB Human
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Immunogen
Synthetic peptide within Human ZAP70 (phospho Y493). The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
Database link: P43403 -
Positive control
- WB: Jurkat (treated with pervanadate) cell extract. Flow cyt: Jurkat and NIH/3T3 cells (both treated with pervanadate).
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.02% Sodium azide
Constituents: 49.88% PBS, 50% Glycerol (glycerin, glycerine), 0.1% BSA -
Concentration information loading... -
Purity
Protein A/G purified -
Clonality
Monoclonal -
Clone number
Zap70Y493-H11 -
Isotype
IgG -
Light chain type
kappa -
Research areas
Images
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Flow Cytometry - Anti-Syk (phospho Y526) + ZAP70 (phospho Y493) antibody [Zap70Y493-H11] (ab278675)Flow cytometric analysis of NIH3T3 cells secondary antibody only negative control (blue) or 0.1 µg/mL of isotype control (orange) or treated with imatinib (red) or with pervanadate (green) using ab278675 at 0.1 µg/mL.
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![Western blot - Anti-Syk (phospho Y526) + ZAP70 (phospho Y493) antibody [Zap70Y493-H11] (ab278675)](https://www.abcam.com/ps/products/278/ab278675/Images/ab278675-453688-anti-syk-western-blot-jurkat-human.jpg)
Western blot - Anti-Syk (phospho Y526) + ZAP70 (phospho Y493) antibody [Zap70Y493-H11] (ab278675)All lanes : Anti-Syk (phospho Y526) + ZAP70 (phospho Y493) antibody [Zap70Y493-H11] (ab278675) at 1 µg/ml
Lane 1 : Jurkat cell extract treated with pervanadate
Lane 2 : Jurkat cell extract untreated
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![Flow Cytometry - Anti-Syk (phospho Y526) + ZAP70 (phospho Y493) antibody [Zap70Y493-H11] (ab278675)](https://www.abcam.com/ps/products/278/ab278675/Images/ab278675-2-zapy493h11.jpg)
Flow Cytometry - Anti-Syk (phospho Y526) + ZAP70 (phospho Y493) antibody [Zap70Y493-H11] (ab278675)Flow cytometric analysis of Jurkat cells secondary antibody only negative control (blue) or untreated (red) or treated with pervanadate (green) using ab278675.
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![Anti-Syk (phospho Y526) + ZAP70 (phospho Y493) antibody [Zap70Y493-H11] (ab278675)](https://www.abcam.com/ps/products/278/ab278675/Images/ab278675-1-benefits-of-recombinant-antibodies.png)
Anti-Syk (phospho Y526) + ZAP70 (phospho Y493) antibody [Zap70Y493-H11] (ab278675)