Anti-STX17 antibody (ab229646)
Key features and details
- Rabbit polyclonal to STX17
- Suitable for: WB, ICC/IF, IHC-P
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-STX17 antibody
See all STX17 primary antibodies -
Description
Rabbit polyclonal to STX17 -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IF, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Recombinant fragment within Human STX17 (internal sequence). The exact sequence is proprietary.
Database link: P56962 -
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-STX17 antibody (ab229646)All lanes : Anti-STX17 antibody (ab229646) at 1/1000 dilution
Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 2 : A431 (human epidermoid carcinoma cell line) whole cell lysate
Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 4 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG antibody
Predicted band size: 33 kDa12% SDS-PAGE
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STX17 antibody (ab229646)Paraffin-embedded mouse prostate tissue staining STX17 using ab229646 at 1/500 dilution in immunohistochemical analysis.
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Immunocytochemistry/ Immunofluorescence - Anti-STX17 antibody (ab229646)Immunofluorescence analysis of 4% paraformaldehyde-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell labeling STX17 (green) with ab229646 at 1/500 dilution. Blue: Fluoroshield with DAPI. Scale bar= 10 μm.
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Western blot - Anti-STX17 antibody (ab229646)All lanes : Anti-STX17 antibody (ab229646) at 1/5000 dilution
Lane 1 : Non-transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 2 : Transfected HEK-293T whole cell lysate
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG antibody
Predicted band size: 33 kDa12% SDS-PAGE
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Immunocytochemistry/ Immunofluorescence - Anti-STX17 antibody (ab229646)Immunofluorescence analysis of 4% paraformaldehyde-fixed DIV9 rat E18 primary cortical neurons labeling STX17 (green) with ab229646 at 1/500 dilution. Red: beta Tubulin 3, using beta Tubulin 3 antibody at 1/500 dilution. Blue: DAPI.
