Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-STAT4 antibody [EP1900Y] (ab68156)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EP1900Y] to STAT4
  • Suitable for: WB, IP, IHC-P, ICC, Flow Cyt
  • Reacts with: Human

Overview

  • Product name

    Anti-STAT4 antibody [EP1900Y]
    See all STAT4 primary antibodies

  • Description

    Rabbit monoclonal [EP1900Y] to STAT4

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC
    Human
    IHC-P
    Human
    IP
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide within Human STAT4 aa 700 to the C-terminus. The exact sequence is proprietary.
    Database link: Q14765

  • Positive control

    • WB: Daudi, K562 and Hela cell lysates. IHC-P: Human tonsil tissue sections. ICC: HeLa cells Flow Cyt: K562 cells IP: HeLa whole cell lysate.

  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STAT4 antibody [EP1900Y] (ab68156)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STAT4 antibody [EP1900Y] (ab68156)

    ab68156, at 1/100 dilution, staining STAT4 in paraffin embedded human tonsil tissue sections by Immunohistochemistry

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Western blot - Anti-STAT4 antibody [EP1900Y] (ab68156)
    Western blot - Anti-STAT4 antibody [EP1900Y] (ab68156)
    All lanes : Anti-STAT4 antibody [EP1900Y] (ab68156) at 1/1000 dilution

    Lane 1 : Daudi cell lysate
    Lane 2 : K562 cell lysate
    Lane 3 : Hela cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size: 86 kDa
    Observed band size: 86 kDa
    Additional bands at: 30 kDa. We are unsure as to the identity of these extra bands.

  • Immunocytochemistry - Anti-STAT4 antibody [EP1900Y] (ab68156)
    Immunocytochemistry - Anti-STAT4 antibody [EP1900Y] (ab68156)
    Immunocytochemistry analysis of HeLa (human cervix adenocarcinoma epithelial cell) labeling STAT4 with purified ab68156 at 1/50 dilution (9 µg/ml). Cells were fixed with 4% Paraformaldehyde and permeabilised with 0.1% tritonX-100. Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/1000 (2 µg/ml) was used as the secondary antibody. ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.16 µg/ml) was used as counterstain. Nuclei were stained blue with DAPI.
    Negative control: PBS instead of the primary antibody.
  • Immunoprecipitation - Anti-STAT4 antibody [EP1900Y] (ab68156)
    Immunoprecipitation - Anti-STAT4 antibody [EP1900Y] (ab68156)

    Purified ab68156 at 1/30 dilution (2µg) immunoprecipitating STAT4 in HeLa whole cell lysate.
    Lane 1 (input): HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate 10µg
    Lane 2 (+): ab68156 + HeLa whole cell lysate.
    Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab68156 in HeLa whole cell lysate.
    VeriBlot for IP Detection Reagent (HRP) (ab131366) (1/1000 dilution) was used for Western blotting.
    Blocking Buffer and concentration: 5% NFDM/TBST.
    Diluting buffer and concentration: 5% NFDM/TBST.
    Observed band size: 86 kDa

  • Flow Cytometry - Anti-STAT4 antibody [EP1900Y] (ab68156)
    Flow Cytometry - Anti-STAT4 antibody [EP1900Y] (ab68156)
    Flow Cytometry analysis of K562 (Human chronic myelogenous leukemia lymphoblast) cells labeling STAT4 with purified ab68156 at 1/100 dilution (10 µg/mL) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cells without incubation with primary antibody and secondary antibody (Blue).
  • Anti-STAT4 antibody [EP1900Y] (ab68156)
    Anti-STAT4 antibody [EP1900Y] (ab68156)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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