Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-STAT3 antibody [84/Stat3] - BSA and Azide free (ab280219)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Mouse monoclonal [84/Stat3] to STAT3 - BSA and Azide free
  • Suitable for: WB
  • Knockout validated
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-STAT3 antibody [84/Stat3] - BSA and Azide free
    See all STAT3 primary antibodies

  • Description

    Mouse monoclonal [84/Stat3] to STAT3 - BSA and Azide free

  • Host species

    Mouse

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    WB
    Mouse
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Wild-type HeLa whole cell lysate. K562, THP-1, NIH/3T3, PC-12, RAW264.7 and Jurkat whole cell lysate.

  • General notes

    This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

    ab280219 is the carrier-free version of ab280212. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab280219 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

Properties

Images

  • Western blot - Anti-STAT3 antibody [84/Stat3] - BSA and Azide free (ab280219)
    Western blot - Anti-STAT3 antibody [84/Stat3] - BSA and Azide free (ab280219)
    All lanes : Anti-STAT3 antibody [84/Stat3] (ab280212) at 1/1000 dilution

    Lane 1 : Wild-type HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate
    Lane 2 : Stat3 knockout HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate
    Lane 3 : K562 (human chronic myelogenous leukemia lymphoblast), whole cell lysate
    Lane 4 : Jurkat (human T cell leukemia T lymphocyte), whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Mouse IgG H&L (IRDye® 800CW) (ab216772) and Goat Anti-Rabbit IgG H&L (IRDye® 680RD) (ab216777) at 1/10000 dilution

    Predicted band size: 88 kDa



    This image was produced using ab280212, the same clone in a different formulation.

    Lanes 1 - 4: Merged signal (red and green). Green - ab280212 observed at 88 kDa. Red - loading control ab181602(Rabbit monoclonal [EPR16891] to GAPDH) observed at 36 kDa.

    Lanes 1-2: ab280212 Anti-Stat3 antibody was shown to react with in HeLa cells in Western blot. Loss of signal was observed when Stat3 knockout sample was used. Wild-type and Stat3 knockout samples were subjected to SDS-PAGE.

    ab280212 and Anti-GAPDH antibody [EPR16891] (ab181602) were incubated at 4? overnight at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 680CW) (ab216777) and Goat anti-Mouse IgG H&L (IRDye® 800RD) (ab216772) secondary antibodies at 1 in 10000 dilution for 1 hour at room temperature before imaging.

    Blocking/Dilution buffer: Intercept® (TBS) Blocking Buffer diluted with an equal volume of 0.1% TBS.

  • Western blot - Anti-STAT3 antibody [84/Stat3] - BSA and Azide free (ab280219)
    Western blot - Anti-STAT3 antibody [84/Stat3] - BSA and Azide free (ab280219)
    All lanes : Anti-STAT3 antibody [84/Stat3] (ab280212) at 1/1000 dilution

    Lane 1 : RAW264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage), whole cell lysate
    Lane 2 : THP-1 (human monocytic leukemia monocyte), whole cell lysate
    Lane 3 : Jurkat (human T cell leukemia T lymphocyte), whole cell lysate
    Lane 4 : HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate
    Lane 5 : NIH/3T3 (mouse embryonic fibroblast), whole cell lysate
    Lane 6 : PC-12 (rat adrenal gland pheochromocytoma), whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution

    Predicted band size: 88 kDa



    This image was produced using ab280212, the same clone in a different formulation.

    Lysates should be made freshly and used in WB immediately to minimize protein degradation.

    Blocking/Dilution buffer: 5% NFDM/TBST.

    Exposure times: Lane 1/4-6: 37 secs;

    Lanes 2-3: 15 secs.

  • Anti-STAT3 antibody [84/Stat3] - BSA and Azide free (ab280219)
    Anti-STAT3 antibody [84/Stat3] - BSA and Azide free (ab280219)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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