Antibodies, Proteins, Kits and Reagents for Life Science

257 980 ₸ Product size

100 µl 257 980 ₸

Order now and get it on Friday March 05, 2021

Anti-STARD4 antibody [EPR17847] (ab200344)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR17847] to STARD4
Suitable for: WB, ICC/IF
Reacts with: Human

Product name

Anti-STARD4 antibody [EPR17847]
See all STARD4 primary antibodies
Description

Rabbit monoclonal [EPR17847] to STARD4
Host species

Rabbit
Tested Applications & Species

Application Species
ICC/IF
Human

WB
Human

See all applications and species data
Immunogen

Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
Positive control
- SH-SY5Y, HepG2, 293 and U-937 whole cell lysate. Human fetal heart, fetal kidney and fetal liver lysate. SH-SY5Y cells.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Application	Species
ICC/IF	Human
WB	Human

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR17847
Isotype

IgG
Research areas

Immunocytochemistry/ Immunofluorescence - Anti-STARD4 antibody [EPR17847] (ab200344)

Immunocytochemistry/ Immunofluorescence analysis of SH-SY5Y (Human neuroblastoma from bone marrow cells) cells labeling STARD4 with ab200344 at a 1/100 dilution. ab150077 AlexaFluor®488 Goat anti-Rabbit secondary, 1/500 dilution. Counterstained using ab7291 anti-Tubulin (mouse mAb), 1/1000 dilution and ab150120 AlexaFluor®594 Goat anti-Mouse secondary, 1/500 dilution. Cells were fixed in 100% methanol and permeabilized using 0.1% Triton X-100. Nuclear stain DAPI.
Western blot - Anti-STARD4 antibody [EPR17847] (ab200344)

All lanes : Anti-STARD4 antibody [EPR17847] (ab200344) at 1/1000 dilution

Lane 1 : SH-SY5Y (Human neuroblastoma from bone marrow cells) whole cell lysate
Lane 2 : HepG2 (Human liver hepatocellular carcinoma) whole cell lysate
Lane 3 : 293 (Human epithelial cells from embryonic kidney) whole cell lysate
Lane 4 : U-937 (Human histiocytic lymphoma cells) whole cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 24 kDa
Western blot - Anti-STARD4 antibody [EPR17847] (ab200344)

All lanes : Anti-STARD4 antibody [EPR17847] (ab200344) at 1/1000 dilution

Lane 1 : Human fetal heart lysate
Lane 2 : Human fetal kidney lysate
Lane 3 : Human fetal liver lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

Predicted band size: 24 kDa
Anti-STARD4 antibody [EPR17847] (ab200344)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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