Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Stanniocalcin 2/STC-2 antibody [EPR22866-10] - BSA and Azide free (ab261915)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR22866-10] to Stanniocalcin 2/STC-2 - BSA and Azide free
  • Suitable for: WB, IHC-P, ICC/IF, Flow Cyt, IP
  • Reacts with: Human

Overview

  • Product name

    Anti-Stanniocalcin 2/STC-2 antibody [EPR22866-10] - BSA and Azide free
    See all Stanniocalcin 2/STC-2 primary antibodies

  • Description

    Rabbit monoclonal [EPR22866-10] to Stanniocalcin 2/STC-2 - BSA and Azide free

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    IHC-P
    Human
    IP
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HeLa, HepG2, MCF7, HT-29, 293T, HCT116 and Human breast cancer lysates. IHC-P: Human lung cancer and Human breast cancer tissues. ICC/IF: T-47D and HepG2 cells. Flow Cyt: T-47D and HepG2 cells. IP: T-47D cells.

  • General notes

    Ab261915 is the carrier-free version of ab255610. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab261915 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

Images

  • Immunoprecipitation - Anti-Stanniocalcin 2/STC-2 antibody [EPR22866-10] - BSA and Azide free (ab261915)
    Immunoprecipitation - Anti-Stanniocalcin 2/STC-2 antibody [EPR22866-10] - BSA and Azide free (ab261915)

    STC2 was immunoprecipitated from 0.35 mg T-47D (human ductal breast epithelial tumor epithelial cell) whole cell lysate with ab255610 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab255610 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366) was used at 1/5000 dilution.

    Lane 1: T-47D (human ductal breast epithelial tumor epithelial cell) whole cell lysate 10ug

    Lane 2: ab255610 IP in T-47D whole cell lysate

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab255610 in T-47D whole cell lysate

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 30 seconds.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab255610).

  • Flow Cytometry - Anti-Stanniocalcin 2/STC-2 antibody [EPR22866-10] - BSA and Azide free (ab261915)
    Flow Cytometry - Anti-Stanniocalcin 2/STC-2 antibody [EPR22866-10] - BSA and Azide free (ab261915)

    Flow cytometric analysis of 4% paraformaldehyde fixed, 90% methanol permeabilized HepG2 (human hepatocellular carcinoma epithelial cell) cells labelling STC2 with ab255610 at 1/50 dilution (Red) compared with a Rabbit monoclonal IgG (ab172730) / Black isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab255610).

  • Flow Cytometry - Anti-Stanniocalcin 2/STC-2 antibody [EPR22866-10] - BSA and Azide free (ab261915)
    Flow Cytometry - Anti-Stanniocalcin 2/STC-2 antibody [EPR22866-10] - BSA and Azide free (ab261915)

    Flow cytometric analysis of 4% paraformaldehyde fixed, 90% methanol permeabilized T-47D (human ductal breast epithelial tumor epithelial cell) cells labelling STC2 with ab255610 at 1/500 dilution (Red) compared with a Rabbit monoclonal IgG (ab172730) / Black isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab255610).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Stanniocalcin 2/STC-2 antibody [EPR22866-10] - BSA and Azide free (ab261915)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Stanniocalcin 2/STC-2 antibody [EPR22866-10] - BSA and Azide free (ab261915)

    Immunohistochemical analysis of paraffin-embedded Human breast cancer (A) and its adjacent noncancerous breast tissue (B) tissue labeling STC2 with ab255610 at 1/ 200 dilution (2.29ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Strong cytoplasmic staining in human breast cancer tissue (A) while weak staining in its adjacent noncancerous breast tissue (B) (PMID: 18492817) is observed. The section was incubated with ab255610 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab255610).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Stanniocalcin 2/STC-2 antibody [EPR22866-10] - BSA and Azide free (ab261915)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Stanniocalcin 2/STC-2 antibody [EPR22866-10] - BSA and Azide free (ab261915)

    Immunohistochemical analysis of paraffin-embedded Human lung cancer (A) and its adjacent noncancerous lung tissue (B) tissue labeling STC2 with ab255610 at 1/ 200 dilution (2.29ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Strong cytoplasmic staining in human lung cancer tissue (A) while weak staining in its adjacent noncancerous lung tissue (B) (PMID: 25463045) is observed. The section was incubated with ab255610 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins. 

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab255610).

  • Immunocytochemistry/ Immunofluorescence - Anti-Stanniocalcin 2/STC-2 antibody [EPR22866-10] - BSA and Azide free (ab261915)
    Immunocytochemistry/ Immunofluorescence - Anti-Stanniocalcin 2/STC-2 antibody [EPR22866-10] - BSA and Azide free (ab261915)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized HepG2 (human hepatocellular carcinoma epithelial cell) cells labelling STC2 with ab255610 at 1/50 (9 ug/ml) dilution, followed by ab150077 AlexaFluor® 488 Goat anti-Rabbit secondary antibody at 1/1000 (2 ug/ml) dilution (Green). Confocal image showing cytoplasmic staining in HepG2 cell line. Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (Red). The Nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Secondary antibody is ab150077 AlexaFluor® 488 Goat anti-Rabbit secondary at 1/1000 (2 ug/ml) dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab255610).

  • Immunocytochemistry/ Immunofluorescence - Anti-Stanniocalcin 2/STC-2 antibody [EPR22866-10] - BSA and Azide free (ab261915)
    Immunocytochemistry/ Immunofluorescence - Anti-Stanniocalcin 2/STC-2 antibody [EPR22866-10] - BSA and Azide free (ab261915)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized T-47D (human ductal breast epithelial tumor epithelial cell) cells labelling STC2 with ab255610 at 1/50 (9 ug/ml) dilution, followed by ab150077 AlexaFluor® 488 Goat anti-Rabbit secondary antibody at 1/1000 (2 ug/ml) dilution (Green). Confocal image showing cytoplasmic staining in T-47D cell line. Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200  dilution (Red). The Nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Secondary antibody is Ab255610 anti-STC2 ab150077 AlexaFluor® 488 Goat anti-Rabbit secondary at 1/1000 (2 ug/ml) dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab255610).

  • Anti-Stanniocalcin 2/STC-2 antibody [EPR22866-10] - BSA and Azide free (ab261915)
    Anti-Stanniocalcin 2/STC-2 antibody [EPR22866-10] - BSA and Azide free (ab261915)

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