Anti-SPT5 antibody [EPR5145(2)] (ab126592)
![Anti-SPT5 antibody [EPR5145(2)] (ab126592)](https://www.abcam.com/ps/products/126/ab126592/Images/ab126592-140537-anti-spt5-antibody-epr51452-western-blot.jpg "Anti-SPT5 antibody [EPR5145(2)] (ab126592)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR5145(2)] to SPT5
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-SPT5 antibody [EPR5145(2)]
See all SPT5 primary antibodies -
Description
Rabbit monoclonal [EPR5145(2)] to SPT5 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF HumanIHC-P HumanWB Human
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Immunogen
Synthetic peptide within Human SPT5 aa 750-850. The exact sequence is proprietary.
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Positive control
- HeLa, HepG2, RAW264.7, PC12, NIH3T3, Jurkat and 293T cell lysates; Human colon tissue; HeLa cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Dissociation constant (KD)
KD = 5.31 x 10 -11 M Learn more about KD -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR5145(2) -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-SPT5 antibody [EPR5145(2)] (ab126592)All lanes : Anti-SPT5 antibody [EPR5145(2)] (ab126592) at 1/1000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : HepG2 cell lysate
Lane 3 : RAW264.7 cell lysate
Lane 4 : PC12 cell lysate
Lane 5 : NIH 3T3 cell lysate
Lane 6 : Jurkat cell lysate
Lane 7 : 293T cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 121 kDa
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SPT5 antibody [EPR5145(2)] (ab126592)](https://www.abcam.com/ps/products/126/ab126592/Images/ab126592-140536-anti-spt5-antibody-epr51452-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SPT5 antibody [EPR5145(2)] (ab126592)ab126592 at 1/100 dilution staining SPT5 in paraffin-embedded Human colon tissue by immunohistochemistry.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Immunocytochemistry/ Immunofluorescence - Anti-SPT5 antibody [EPR5145(2)] (ab126592)](https://www.abcam.com/ps/products/126/ab126592/Images/ab126592-140535-anti-spt5-antibody-epr51452-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-SPT5 antibody [EPR5145(2)] (ab126592)ab126592 at 1/100 dilution staining SPT5 in HeLa cells by immunofluorescence.
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![OI-RD Scanning - Anti-SPT5 antibody [EPR5145(2)] (ab126592)](https://www.abcam.com/ps/products/126/ab126592/Images/ab126592-140534-anti-spt5-antibody-epr51452.jpg)
OI-RD Scanning - Anti-SPT5 antibody [EPR5145(2)] (ab126592) -
![Anti-SPT5 antibody [EPR5145(2)] (ab126592)](https://www.abcam.com/ps/products/126/ab126592/Images/ab126592-5-benefits-of-recombinant-antibodies.png)
Anti-SPT5 antibody [EPR5145(2)] (ab126592)
