Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-SPT5 antibody [EPR5145(2)] (ab126592)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR5145(2)] to SPT5
  • Suitable for: WB, IHC-P, ICC/IF
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-SPT5 antibody [EPR5145(2)]
    See all SPT5 primary antibodies

  • Description

    Rabbit monoclonal [EPR5145(2)] to SPT5

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide within Human SPT5 aa 750-850. The exact sequence is proprietary.

  • Positive control

    • HeLa, HepG2, RAW264.7, PC12, NIH3T3, Jurkat and 293T cell lysates; Human colon tissue; HeLa cells.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.

  • Dissociation constant (KD)

    KD = 5.31 x 10 -11 M
    Learn more about KD

  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...

  • Purity

    Tissue culture supernatant

  • Clonality

    Monoclonal

  • Clone number

    EPR5145(2)

  • Isotype

    IgG

  • Research areas

Images

  • Western blot - Anti-SPT5 antibody [EPR5145(2)] (ab126592)
    Western blot - Anti-SPT5 antibody [EPR5145(2)] (ab126592)
    All lanes : Anti-SPT5 antibody [EPR5145(2)] (ab126592) at 1/1000 dilution

    Lane 1 : HeLa cell lysate
    Lane 2 : HepG2 cell lysate
    Lane 3 : RAW264.7 cell lysate
    Lane 4 : PC12 cell lysate
    Lane 5 : NIH 3T3 cell lysate
    Lane 6 : Jurkat cell lysate
    Lane 7 : 293T cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat anti-rabbit HRP at 1/2000 dilution

    Predicted band size: 121 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SPT5 antibody [EPR5145(2)] (ab126592)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SPT5 antibody [EPR5145(2)] (ab126592)

    ab126592 at 1/100 dilution staining SPT5 in paraffin-embedded Human colon tissue by immunohistochemistry.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunocytochemistry/ Immunofluorescence - Anti-SPT5 antibody [EPR5145(2)] (ab126592)
    Immunocytochemistry/ Immunofluorescence - Anti-SPT5 antibody [EPR5145(2)] (ab126592)

    ab126592 at 1/100 dilution staining SPT5 in HeLa cells by immunofluorescence.

  • OI-RD Scanning - Anti-SPT5 antibody [EPR5145(2)] (ab126592)
    OI-RD Scanning - Anti-SPT5 antibody [EPR5145(2)] (ab126592)
    Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD
  • Anti-SPT5 antibody [EPR5145(2)] (ab126592)
    Anti-SPT5 antibody [EPR5145(2)] (ab126592)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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