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Epigenetics and Nuclear Signaling Transcription Domain Families Zinc Finger

Anti-splicing factor 1 antibody (ab228535)

Anti-splicing factor 1 antibody (ab228535)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Rabbit polyclonal to splicing factor 1
  • Suitable for: WB, ICC/IF
  • Reacts with: Human
  • Isotype: IgG

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Overview

  • Product name

    Anti-splicing factor 1 antibody
    See all splicing factor 1 primary antibodies
  • Description

    Rabbit polyclonal to splicing factor 1
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    WB
    Human
    See all applications and species data
  • Immunogen

    Recombinant fragment within Human splicing factor 1 (internal sequence). The exact sequence is proprietary.
    Database link: Q15637

  • Positive control

    • WB: Raji whole cell lysate. ICC/IF: A-375 cells.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine)
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Epigenetics and Nuclear Signaling
    • Transcription
    • Domain Families
    • Zinc Finger
    • Epigenetics and Nuclear Signaling
    • DNA / RNA
    • RNA Processing
    • Splicing
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Transcription Factors

Images

  • Western blot - Anti-splicing factor 1 antibody (ab228535)
    Western blot - Anti-splicing factor 1 antibody (ab228535)
    Anti-splicing factor 1 antibody (ab228535) at 1/1000 dilution + Raji (human Burkitt's lymphoma cell line) whole cell lysate at 30 µg

    Developed using the ECL technique.

    Predicted band size: 68 kDa



    7.5% SDS-PAGE

  • Immunocytochemistry/ Immunofluorescence - Anti-splicing factor 1 antibody (ab228535)
    Immunocytochemistry/ Immunofluorescence - Anti-splicing factor 1 antibody (ab228535)

    A-375 (human malignant melanoma cell line) cells stained for splicing factor 1 (green) using ab228535 at 1/500 dilution in ICC/IF. Cells were fixed in 4% paraformaldehyde at RT for 15 minutes.

    Blue: Hoechst 33342 staining.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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