Antibodies, Proteins, Kits and Reagents for Life Science

251 280 ₸ Product size

100 µl 251 280 ₸

Order now and get it on Wednesday March 10, 2021

Anti-SPINK1/P12 antibody [EPR17585-30] (ab206294)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR17585-30] to SPINK1/P12
Suitable for: IHC-P, WB
Reacts with: Human

Product name

Anti-SPINK1/P12 antibody [EPR17585-30]
See all SPINK1/P12 primary antibodies
Description

Rabbit monoclonal [EPR17585-30] to SPINK1/P12
Host species

Rabbit
Tested Applications & Species

Application Species
WB
Human

See all applications and species data
Immunogen

This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB; Human pancreas lysate. IHC-P; Human pancreatic carcinoma tissue, Human liver carcinoma tissue.
General notes
This product was previously labelled as SPINK1

This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

Learn more here.

Application	Species
WB	Human

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR17585-30
Isotype

IgG
Research areas

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SPINK1/P12 antibody [EPR17585-30] (ab206294)

Immunohistochemical analysis of paraffin-embedded human pancreatic carcinoma tissue labeling SPINK1/P12 with ab206294 at 1/100 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Cytoplasmic staining on human pancreatic carcinoma tissue is observed.

Counter stained with Hematoxylin.
Negative control: Used PBS instead of primary ab, secondary ab is Goat Anti-Rabbit IgG H&L (HRP) (ab97051).

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SPINK1/P12 antibody [EPR17585-30] (ab206294)

Immunohistochemical analysis of paraffin-embedded human liver carcinoma tissue labeling SPINK1/P12 with ab206294 at 1/100 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Cytoplasmic staining on human liver carcinoma tissue is observed.

Counter stained with Hematoxylin.
Negative control: Used PBS instead of primary ab, secondary ab is Goat Anti-Rabbit IgG H&L (HRP) (ab97051).

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Western blot - Anti-SPINK1/P12 antibody [EPR17585-30] (ab206294)

All lanes : Anti-SPINK1/P12 antibody [EPR17585-30] (ab206294) at 1/5000 dilution

Lane 1 : Human pancreas lysate
Lane 2 : Human fetal kidney
Lane 3 : HepG2 (Human liver hepatocellular carcinoma cell line) whole cell lysate
Lane 4 : BxPC-3 (Human pancreas adenocarcinoma cell line) whole cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/10000 dilution

Predicted band size: 8 kDa
Observed band size: 11 kDa
why is the actual band size different from the predicted?

Blocking/dilution buffer: 5% NFDM/TBST.
Exposure time; Lane 1:5 seconds;
Lane 2,3 and 4: 3 minutes.
Anti-SPINK1/P12 antibody [EPR17585-30] (ab206294)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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