Anti-SPINK1/P12 antibody [EPR17585-116] - BSA and Azide free (ab251469)
![Anti-SPINK1/P12 antibody [EPR17585-116] - BSA and Azide free (ab251469)](https://www.abcam.com/ps/products/251/ab251469/Images/ab251469-3-anti-spink1-p12-antibody-epr17585-116-bsa-and-azide-free-immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections.jpg "Anti-SPINK1/P12 antibody [EPR17585-116] - BSA and Azide free (ab251469)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR17585-116] to SPINK1/P12 - BSA and Azide free
- Suitable for: WB, IHC-P
- Reacts with: Human
Overview
-
Product name
Anti-SPINK1/P12 antibody [EPR17585-116] - BSA and Azide free
See all SPINK1/P12 primary antibodies -
Description
Rabbit monoclonal [EPR17585-116] to SPINK1/P12 - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant full length protein. This information is proprietary to Abcam and/or its suppliers.
-
General notes
ab251469 is the carrier-free version of ab207302 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
Ab251469 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product was previously labelled as SPINK1
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Clonality
Monoclonal -
Clone number
EPR17585-116 -
Isotype
IgG -
Research areas
Images
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SPINK1/P12 antibody [EPR17585-116] - BSA and Azide free (ab251469)This data was developed using ab207302, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded human pancreas tissue labeling SPINK1/P12 with ab207302 at 1/200 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasmic staining on human pancreas is observed. Counter stained with Hematoxylin. Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SPINK1/P12 antibody [EPR17585-116] - BSA and Azide free (ab251469)](https://www.abcam.com/ps/products/251/ab251469/Images/ab251469-2-anti-spink1-p12-antibody-epr17585-116-bsa-and-azide-free-immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SPINK1/P12 antibody [EPR17585-116] - BSA and Azide free (ab251469)This data was developed using ab207302, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded human hepatocellular carcinoma tissue labeling SPINK1/P12 with ab207302 at 1/200 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasmic staining on hepatocellular carcinoma is observed. Counter stained with Hematoxylin. Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. -
![Western blot - Anti-SPINK1/P12 antibody [EPR17585-116] - BSA and Azide free (ab251469)](https://www.abcam.com/ps/products/251/ab251469/Images/ab251469-399212-anti-spink1-p12-antibody-epr17585-116-bsa-and-azide-free-western-blot.jpg)
Western blot - Anti-SPINK1/P12 antibody [EPR17585-116] - BSA and Azide free (ab251469)All lanes : Anti-SPINK1/P12 antibody [EPR17585-116] (ab207302) at 1/5000 dilution
Lane 1 : Human pancreas lysate
Lane 2 : Human fetal kidney lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/10000 dilution
Predicted band size: 8 kDa
Observed band size: 11 kDa why is the actual band size different from the predicted?This data was developed using ab207302, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
Exposure time: Lane 1: 1 minute; Lane 2: 3 minutes.
SPINK1/P12 is a pancreatic secretory trypsin inhibitor, uniquely expressed in pancreas. Human fetal kidney lysate is used as negative control.
SPINK1/P12 contains a signal peptide, thus observed with a MW of 11 kDa (PMCID: PMC2765331).
-
![Anti-SPINK1/P12 antibody [EPR17585-116] - BSA and Azide free (ab251469)](https://www.abcam.com/ps/products/251/ab251469/Images/ab251469-4-benefits-of-recombinant-antibodies.png)
Anti-SPINK1/P12 antibody [EPR17585-116] - BSA and Azide free (ab251469)
